The tumors were fixed in 4% (w/v) formaldehyde solution for 24 h followed by storage in 70% ethanol. The fixed tumors were processed, paraffin-embedded, and cut at 4 µm thick slices. The sections were stained for cleaved caspase-3 (for apoptosis), Ki-67 (for tumor proliferation), and CD31 (for angiogenesis). Both cleaved-caspase 3 (Cell Signaling Technology, Danvers, MA, USA) and Ki-67 clone SP-6 (Biocare, Concord, CA, USA) utilized a 1:100 antibody concentration followed by Envision Rabbit Horseradish Peroxidase (HRP) detection system (Dako, Carpenteria, CA, USA). CD31assay utilized a 1:1200 antibody concentration (Santa Cruz, Dallas, TX, USA) followed by Goat-on-Rodent (HRP) polymer system (Biocare, Concord, CA, USA). All sections were developed using DAB chromogen (Dako) and counterstained with Mayer’s Hematoxylin (Dako). The relative staining for each target was analyzed by ImageJ software (Bethesda, MD, USA) to determine the fraction of positive stain per unit tissue area.
Goat on rodent hrp polymer system
Manufactured by Biocare Medical
Sourced in United States
The Goat-on-Rodent (HRP) polymer system is a laboratory equipment product designed for use in immunohistochemical and immunocytochemical applications. The core function of this system is to provide a detection method that amplifies the signal from the primary antibody, thereby enhancing the visualization of target antigens in tissue or cell samples.
Automatically generated - may contain errors
Lab products found in correlation
Ki 67 clone sp 6, by Biocare Medical (2 mentions)
Dab chromogen, by Agilent Technologies (2 mentions)
Envision rabbit horseradish peroxidase hrp detection system, by Agilent Technologies (2 mentions)
Mayer s hematoxylin, by Agilent Technologies (2 mentions)
Cleaved caspase 3, by Cell Signaling Technology (2 mentions)
2 protocols using goat on rodent hrp polymer system
1
Quantification of Tumor Biomarkers
2
Immunohistochemical Analysis of Lung Tumors
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Lung tumors from the therapeutic study were fixed in 4% w/v formaldehyde solution for 24 h and subsequently transferred to 70% (v/v) ethanol. Tissue samples were embedded in paraffin and sectioned into 4 μm-thick slices. The sections were deparaffinized and stained for cleaved caspase-3, Ki67, and CD31. Both cleaved-caspase 3 (Cell Signaling Technology, Danvers, MA, USA) and Ki-67 clone SP-6 (Biocare Medical, Concord, CA, USA) staining used a 1:100 antibody concentration followed by Envision Rabbit Horseradish Peroxidase (HRP) detection system (Dako, Carpinteria, CA, USA). CD31 assay used a 1:1200 antibody concentration (Santa Cruz Biotechnology, Santa Cruz, CA, USA) followed by Goat-on-Rodent HRP polymer system (Biocare Medical). All the sections were developed using DAB chromogen (Dako) and counterstained with Mayer’s Hematoxylin (Dako). The relative staining for each target was analyzed by Image J software (https://imagej.nih.gov/ij/download.html ) to determine the fraction of positive stain per unit tissue area.
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