Ha h3663

Manufactured by Merck Group

Sourced in United States

The HA (H3663) is a laboratory equipment product from Merck Group. It serves as a tool for conducting various experiments and analyses in a research or scientific setting. The core function of this product is to facilitate specific laboratory processes, though the detailed intended use may vary depending on the application and requirements of the user.

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Lab products found in correlation

Horseradish peroxidase conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 594 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Bodipy 493 503, by Thermo Fisher Scientific (1 mentions) 3 isobutyl 1 methyl xanthine (ibmx), by Merck Group (1 mentions) Ab77115, by Abcam (1 mentions) Ab2739, by Abcam (1 mentions) Ab124807, by Abcam (1 mentions) Ab3340, by Abcam (1 mentions) Ab99532, by Abcam (1 mentions) Ab96718, by Abcam (1 mentions) β actin sc 8432, by Santa Cruz Biotechnology (1 mentions) Ab3526, by Abcam (1 mentions) Cl 316 243, by R&D Systems (1 mentions) Hsp90 sc 7947, by Santa Cruz Biotechnology (1 mentions) Jmjd3, by Cell Signaling Technology (1 mentions) Asxl2 a302 037a, by Fortis Life Sciences (1 mentions)

Spelling variants of this product

H3663 (64 citations) Anti-HA (H3663, (8 citations) Anti-HA antibody (H3663; (2 citations) Anti-hemagglutinin (HA)(H3663) (2 citations) Mouse anti-HA (H3663) (2 citations)

4 protocols using ha h3663

Adipocyte Lipid Metabolism Regulation

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Antibodies against OGT (ab96718), OGA (MGEA5, ab124807), O-GlcNAc (RL2, ab2739), PLIN1 (ab3526), SNAP23 (ab3340), ATGL (ab99532), DGAT1 (11561-1-AP), Fsp27 (CIDE C, ab77115), and p-Ser (phosphoserine, PSR-45) (Abcam); p492-phosphorylated PLIN1 (4855) and p517-phosphorylated PLIN1 (4856) (Vala Sciences); HA (H3663) (Sigma-Aldrich); CGI-58 (ABHD5, 12201-1-AP), PLIN2 (15294-1-AP), and PLIN3 (TIP47, 10694-1-AP) (Proteintech); p563-HSL (4139), phospho-Akt (Ser473, 9271), and Akt (9272) (Cell Signaling Technology); Myc (sc-40), DGAT2 (sc-66859), and β-actin (sc-8432) (Santa Cruz Biotechnology) were purchased from the indicated sources. Horseradish peroxidase-conjugated secondary antibodies were from Santa Cruz Biotechnology. Alexa Fluor 594-conjugated secondary antibodies, BODIPY 493/503, and BODIPY 558/568 C12 fatty acid were obtained from Thermo Fisher Scientific. 4′,6-Diamidino-2-phenylindole (DAPI), OA, Fsk, and IBMX were from Sigma-Aldrich. CL-316,243 was from R&D Systems. OGT inhibitor ST045849 (ST04) was purchased from TimTec. TMG was from Cayman Chemical.

Yang Y., Fu M., Li M.D., Zhang K., Zhang B., Wang S., Liu Y., Ni W., Ong Q., Mi J, & Yang X. (2020). O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. Nature Communications, 11, 181.

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Chromatin Remodeling Protein Antibody Panel

Cited 1 time

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BAP1 (For western blot and IP) (#13271S), H3K27me3 (#9733S), SUZ12 (#3737S), EZH2 (#5246S), UTX (#33510), H3K27me3 (#9733), H3K27ac (#8173), H2AK119ub (#8240), H2A (#12349) and JMJD3 (#3457S) antibodies were purchased from Cell Signaling. HSP90 (sc-7947) was purchased from Santa Cruz, M2 Flag (F3165) and HA (H3663) were purchased from Sigma. ASXL2 (A302–037A) and RBBP5 (A300–109A) antibodies were purchased from Bethyl Laboratories. Anti-MLL3 (for western blot and IP) was generated against human MLL3 amino acids, and anti-MLL4 was described previously^{51 (link)}. Anti-BAP1 (for ChIP-seq) was generated against recombinant full-length BAP1 (α-BAP1-FL) or the aa 10–360 of BAP1 protein (α-BAP1-NTD) in house. Anti-MLL3 (for ChIP-seq) was generated against the aa 443–590 (α-MLL3-NTD) or aa 2951–3091 (α-MLL3-MR) of MLL3 protein in house.

Wang L., Zhao Z., Ozark P.A., Fantini D., Marshall S.A., Rendleman E.J., Cozzolino K.A., Louis N., He X., Morgan M.A., Takahashi Y.H., Collings C.K., Smith E.R., Ntziachristos P., Savas J.N., Zou L., Hashizume R., Meeks J.J, & Shilatifard A. (2018). Resetting the Epigenetic Balance of Polycomb and COMPASS Function at Enhancers for Cancer Therapy. Nature medicine, 24(6), 758-769.

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Golgi Complex Protein Localization Protocol

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Mouse anti β-COP G6160 1:1000, Rabbit anti PSMD6 HPA036922 1:1000, Mouse anti HA H3663 1:1000 (Sigma), Rabbit anti Giantin, Rabbit anti TGN46, Mouse anti Giantin ab37266 1:1000, WB 1:100 IF, Giantin ab80864 1:200 IF, Rabbit anti GM130 ab52649 1:5000 WB, 1:200 IF, Rabbit anti PSMD6 ab140461 1:200, IF 1:4000 WB, Rabbit anti PSMD11 ab99414 1:5000, Mouse anti P97/VCP ab11433 |1:2000, Mouse anti 58K-Golgi protein ab27043 1:1000, Mouse anti Grp78 (Bip) ab181499 1:1000, Mouse anti GAPDH AB-AM4300 1:50,000, Rabbit anti Hsp90 ab13495 1:1000, Rabbit anti K48 linked polyubiquitin ab140601-100 1:5000, Rabbit anti Calnexin ab22595 1:1000, Rabbit anti PSMD11 (Cell signaling). Mouse anti alpha-4 SC-271297 1:1000 (Santa Cruz), Mouse anti β-Actin MA5-15739 1:10,000 (invitrogen), Mouse anti alpha-6, produced from hybridoma was a kind gift from Keiji Tanaka. Anti-mouse CD138-APC 561705 1:200 and CD19-PE 115508 1:200 (BD biosciences). Goat anti Mouse 488 A11029 1:400, Goat anti mouse 647 A31571 1:400 (Invitrogen), Goat anti Rabbit 647 AB150075 1:400 (Abcam), Goat anti mouse HRP 115-035-205 1:5000, Goat anti Rabbit HRP | 111-035-003 1:5000 (Jackson labs), K48-TUBE (life-sensors) UM505F 1:50, GFP-GM130 was a kind gift from Martin Lowe, inducible shRNA targeting PSMD6 or luciferase were a kind gift from Yossi Shaul.

Eisenberg-Lerner A., Benyair R., Hizkiahou N., Nudel N., Maor R., Kramer M.P., Shmueli M.D., Zigdon I., Cherniavsky Lev M., Ulman A., Sagiv J.Y., Dayan M., Dassa B., Rosenwald M., Shachar I., Li J., Wang Y., Dezorella N., Khan S., Porat Z., Shimoni E., Avinoam O, & Merbl Y. (2020). Golgi organization is regulated by proteasomal degradation. Nature Communications, 11, 409.

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Characterization of IRF3 and NF-κB Signaling

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Mouse monoclonal antibodies (Flag: F3165 and HA: H3663) were purchased from Sigma-Aldrich (MO, USA). Anti-IRF3 (#4302S), anti-P65 (#8242), and anti-phosphorylated P65 (#3033) were purchased from Cell Signaling Technology (MA, USA). Anti-Myc (sc-40), anti-β actin (sc-1616), anti-β tubulin (sc-55529), and anti-LAMB1 (sc-56144) were purchased from Santa Cruz Biotechnology (TX, USA). Anti-TRAF7 (11780-1-AP) and anti-TBK1 (67211-1-Ig) were purchased from Proteintech (Wuhan, China). Anti-TBK1 (#3504) was purchased from Cell Signaling Technology. Alexa Fluor 647-labeled anti-mouse (A0473), Alexa Fluor 488-labeled anti-rabbit (A0423), and HA tag Rabbit Monoclonal Antibody (AF2305) were purchased from Beyotime (Suzhou, China). SeV and VSV were provided by Dr. Hong-Bing Shu (Wuhan University, China). The mammalian expression plasmids used in this study were previously described (Xu et al., 2004 (link); Ling et al., 2018 (link)).

Huang J.P., Yang Y.X., Chen T., Wang D.D., Li J, & Xu L.G. (2023). TRAF7 negatively regulates the RLR signaling pathway by facilitating the K48-linked ubiquitination of TBK1. Virologica Sinica, 38(3), 419-428.

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