For cell cycle analysis, B-NHL cells were treated with CBL0137 (0.5 μM, 1.0 μM, 1.5 μM, and 2.0 μM) for 24 h and stained with PI/RNase (KeyGEN BioTECH, China) for 30 min. An Annexin V-FITC/PI Apoptosis Kit from Vazyme was used for apoptosis analysis. Flow cytometry was used for detection, and the data were analyzed by FlowJo software.
Annexin 5 fitc pi apoptosis kit
Manufactured by Vazyme
Sourced in China
The Annexin V-FITC/PI Apoptosis Kit is a laboratory equipment product designed for the detection and quantification of apoptosis in cells. It utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide, a fluorescent dye that stains DNA, to identify cells at different stages of the apoptotic process.
Automatically generated - may contain errors
Lab products found in correlation
Facscanto 2, by BD (2 mentions)
Pi rnase, by Keygen Biotech (1 mentions)
Flow cytometry, by Beckman Coulter (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Oxaliplatin, by Selleck Chemicals (1 mentions)
Sodium oxamate, by Selleck Chemicals (1 mentions)
Pka c α, by Cell Signaling Technology (1 mentions)
P creb, by Cell Signaling Technology (1 mentions)
Ly6c apc, by BioLegend (1 mentions)
P h2ax, by Cell Signaling Technology (1 mentions)
Ly6g pe, by BioLegend (1 mentions)
Cyclic amp complete elisa kit, by Abcam (1 mentions)
Glut1, by Cell Signaling Technology (1 mentions)
Cd11b fitc, by BioLegend (1 mentions)
Aldoa, by Cell Signaling Technology (1 mentions)
Pfkfb3, by Cell Signaling Technology (1 mentions)
Pgam1, by Cell Signaling Technology (1 mentions)
Glucose assay kit 1, by Eton Bioscience (1 mentions)
Lactate assay kit 1, by Eton Bioscience (1 mentions)
Caspase 9, by Cell Signaling Technology (1 mentions)
8 protocols using annexin 5 fitc pi apoptosis kit
1
Cell Cycle and Apoptosis Analysis of B-NHL Cells
2
Blue Light-Induced Apoptosis Assay
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Referring to the previously reported method,42 UMUC‐3 and 5637 cells were inoculated into 6‐well plates at 5.0 × 105 cells per well, respectively, and incubated with packaged lentiviruses paCas9‐Survivin and paProtacL‐Survivin separately or in combination for 48 h, and then followed by blue light irradiation (1.2 W/m2). After 48 h of incubation under blue light irradiation, the apoptosis rate in the above‐treated cells was measured by the flow cytometry (Beckman Coulter) via an Annexin V‐FITC/PI Apoptosis Kit (Vazyme) in relevance to the manufacturer's protocol. Each experiment was repeated three times.
3
Quantifying Cellular Apoptosis by Annexin V-FITC/PI Assay
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Cell apoptosis was assessed using an annexin V-FITC/PI apoptosis kit (Vazyme Biotech Co.). A total of 10,000 cells/well were seeded in 6-well plates and treated with germacrone for 48 h. Then, the cells were harvested and washed twice in cold PBS and resuspended in 100 μL binding buffer. The cell suspension was mixed with 5 μL annexin V-FITC and 5 μL PI staining solution for 10 min in the dark at room temperature. The cells were resuspended in 400 μL binding buffer and were analyzed by flow cytometry (Becton Dickinson FACSCanto II, USA) within 1 h.
4
Annexin V-FITC/PI Apoptosis Assay
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An annexin V-FITC/PI apoptosis kit (Vazyme, Nanjing, China) was used to analyse apoptosis. Following transfection for 48 h, 5 μl annexin-FITC and 5 μl PI were added to H1299 and A549 cells for 20 min in the dark at room temperature. Apoptotic cells were analysed within one hour via using a flow cytometer (Becton Dickinson, CA, USA).
5
Quantification of Cell Apoptosis
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The AnnexinV-FITC/PI apoptosis kit (Vazyme Biotech Co.) was used to assess cell apoptosis. Cells were first collected and then washed twice in ice-cold PBS, then resuspended in 100 ul binding buffer. We added 5 μl Annexin V-FITC and 5 μl PI staining solution to the suspension for 10 min at room temperature in the dark. We added 400 μl binding buffer to each sample, followed by detection by flow cytometry (Becton Dickinson FACSCanto II, USA) within 1 h.
6
Apoptosis Assay with Chaetocin
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The Annexin V-FITC/PI Apoptosis Kit was purchased from Vazyme Biotech (Nanjing, China). TE-1 and KYSE150 cells were seeded in 6-well plates at a density of 2 × 106 cells/well, and treated with chaetocin at different concentrations (0, 0.2, 0.4, and 0.8 μM) for 24 h in the presence or absence of NAC (5 mM) or z-VAD-fmk (10 μM) before collection. The cells were analyzed by flow cytometry as previously described [25 (link)].
7
Measuring Apoptosis in Cultured Cells
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After sucking out the culture medium, cells were rinsed with PBS for three times. Then, the cells were digested with the Trypsin (ThermoFisher, #15050065) without EDTA Solution and centrifuged in 1000 g for 5 min. Apoptotic cells stained with Annexin V-FITC/PI apoptosis kit (Vazyme, #A211-01), and subsequently detected using flow cytometry.
8
Metabolic Reprogramming in C. tropicalis
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The C. tropicalis strain (W4162870) was kindly provided by Dr. Sarah L. Gaffen (University of Pittsburgh, PA). Oxaliplatin(S1224), Forskolin (S2449) and Sodium Oxamate (S6871) were purchased from Selleck Chemicals (Houston, Texas, USA). Lactate was purchased from Sigma (St. Louis, MO, USA). Antibody against MLH1 (#3515), MSH2 (#2017), Cleaved caspase3 (#9664), caspase9 (#9508), p-H2AX (Ser139, #9718), PFKFB3 (#13123), LDHA (#3582), PKM2 (#4053), PGAM1 (#12098), GPI (#57893), GLUT1(#12939), ALDOA (#8060), HK2(#2867), PKAC-α(#4782) and p-CREB (Ser133, #9198)were purchased from Cell Signaling Technology (Boston, MO, USA). Antibody against β-tublin (# 66240-1-Ig) was purchased from Proteintech (Wuhan, Hubei, P.R.C). Antibody against GPR81 (YN2554) was purchased from Immunoway (Plano, TX, USA). CCK8 Kit (CK04) was purchased from Dojindo Laboratories (Japan). Annexin V FITC/PI Apoptosis Kit (A211-01) was purchased from Vazyme Biotech (Nanjing, China). F4/80-APC (#123115), CD11b-FITC (#301330), Ly6G-PE (#127607), Ly6C-APC (#128016), Gr-1-FITC (#108405) were all from Biolegend (San Diego, CA, USA). Lactate Assay Kit I (#120001100P) and Glucose Assay Kit I (#120003100P) were purchased from Eton Bioscience (San Diego, USA). Cyclic AMP Complete ELISA Kit (ab133051) was purchased from abcam (Cambridge, England).
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