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22 protocols using ethyl alcohol

1

Toxicity Evaluation of Parabens

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The following chemicals were selected for this study: methyl paraben (purity ≥99.0%) ethyl paraben (≥ 99.0%), n-propyl paraben (≥ 99.0%) and n-butyl paraben (≥ 99.0%) were purchased from Sigma–Aldrich (Saint Quentin, Fallaviers, France). These tested chemicals were special-grade reagents and were used without purification. Stock solutions of each paraben were prepared in ethyl alcohol (purity: 99.9%, Samchun, Korea) for the bioluminescence inhibition test of A. fischeri, and in hard reconstituted water (HRW; 0.12 g/L MgSO4, 0.192 g/L NaHCO3, 0.008 g/L KCl, 0.12 g/L CaCO3 in distilled water) for the acute toxicity test of D. magna.
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2

Potentiometric Ion Sensor Fabrication

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Sodium ionophore X, sodium tetrakis[3,5-bis(trifluoromethyl)phenyl]borate (Na-BARF), bis(2-ethylhexyl) sebacate (DOS), poly(vinyl chloride) (PVC) with high molecular weight, sodium chloride, potassium chloride, magnesium chloride, calcium chloride, ammonium chloride, ethyl cellulose with viscosity 10 cP, terpineol, BUTVAR® B-98 (polyvinyl butyral, PVB), methanol, and tetrahydrofuran (THF) were purchased from Sigma Aldrich. Ethyl alcohol was purchased from Samchun Chemicals Co. Ltd. Ag and Ag/AgCl inks were obtained from Asahi chemical research laboratory Co. Ltd. Deionized (DI) water was prepared by using a Milli-Q unit (resistivity: 18.2 MΩ cm−1, Millipore System, Darmstadt, Germany).
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3

Formulation and Characterization of AmB Liposomes

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AmB was procured from North China Pharmaceutical Huasheng Co. Ltd. (Hebei, China). Hydrogenated soy phosphatidylcholine (HSPC), distearoylphosphatidylglycerol (DSPG), cholesterol, and hydrochloric acid (37%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hanmi Gas Co. Ltd. supplied carbon dioxide with a high purity of 99.9% (Seoul, Republic of Korea). Ethyl alcohol (purity 99.9%), mEthyl alcohol (MeOH, purity 99.5%), chloroform (CHCl3, purity 99.5%), dimethyl sulfoxide (DMSO, purity 99.5%), N,N-dimethylacetamide (DMA, purity 99.5%), and N,N-dimethylformamide (DMF, purity 99.5%) were purchased from Samchun Pure Chemical (Pyeongtaek-si, Republic of Korea). All other chemicals and reagents were of analytical grade. Purified water of Milli-Q quality (Milli-Q Reference, Millipore®, Molshiem, France) was used throughout the study.
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4

Synthesis of Cadmium Selenide Nanocrystals

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Cadmium chloride (CdCl2, 99.99%), selenium (Se, 99.99%), n‐octylamine (OcAm, 99%), trioctylphosphine (TOP, 97%), chloroform (anhydrous, ≥99%), oleylamine (99%), 1‐octadecene (technical grade, 90%), and octane (anhydrous, ≥99%) were purchased from Sigma Aldrich. Ethyl alcohol (anhydrous, 99.9%) was purchased from Samchun Chemicals. n‐Octylammonium hydrochloride was purchased from Tokyo Chemical Industry. CO gas (99.999%) was purchased from Sumitomo Seika Chemicals.
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5

Optimizing Topical Cyclosporine A Formulation

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Restasis® emulsion (Allergan Inc.) was purchased from a local market. CsA was supplied from Concord Drugs Limited (Hyderabad, India). Ethyl alcohol (purity 99.9%) and mEthyl alcohol (purity 99.5%) were purchased from Samchun Pure Chemical Co. Ltd., (Gyeonggi-do, Republic of Korea). A dialysis membrane (molecular weight cut-off: 14,000 Dalton (Da)) from Sigma-Aldrich Co. (St Louis, MO, USA) was used. Purified water of Milli-Q quality (Milli-Q Reference; EMD Millipore, Billerica, MA, USA) was used throughout the study.
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6

Dendropanax morbifera Leaf-Hyaluronate Hydrogel

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The leaves of six-year-old Dendropanax morbifera Léveille and ginsenoside Rh2 (≥90%) were acquired from Ginseng Bank, Kyung Hee University (Yongin, Republic of Korea). High-molecular-weight sodium hyaluronate (HA, MW = 1,280 kDa) was supplied by Xinjiang Fufeng Biotechnologies Co., Ltd. (Xinjiang, China). Zinc nitrate hexahydrate (reagent grade, ≥98%), N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC, reagent grade, ≥98%), 4-(dimethylamino) pyridine (DMAP, ≥99%), hydrochloric acid (HCl, ACS reagent, 37%), and anhydrous N,N-dimethylformamide (DMF, ≥99%) were purchased from Sigma-Aldrich Chemicals (St. Louis, MO, USA). Phosphate buffer (20× PBS) was purchased from Biosesang (Seongnam, Republic of Korea). Trichloroacetic acid (TCA, ≥ 99%), potassium persulfate (≥98%), potassium ferricyanide (≥98%), sodium chloride (NaCl, ≥99.50%), and sodium hydroxide (NaOH, ≥98%) were purchased from Daejung Chemicals and Metals Co., Ltd. (Siheung, Republic of Korea). Glycerol was purchased from Junsei Chemical Co., Ltd (Tokyo, Japan). Iron (III) chloride hexahydrate (97%), methyl alcohol (MeOH, 95%), and ethyl alcohol (EtOH, 95%) were procured from Samchun Pure Chemical Co. Ltd. (Pyeongtaek, Republic of Korea). Other chemicals and reagents were purchased from commercial suppliers.
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7

Synthesis of Chalcogenide Materials

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Antimony(III) sulfide (99.995%. Sigma Aldrich), antimony(III) selenide (99.99%, Sigma Aldrich), tin powder (99.8%, 325 mesh, Acros Organics), selenium powder (99.999%, 200 mesh, Alfa Aesar), sulfur (99.998%, Sigma Aldrich), copper powder (99%, Alfa Aesar), ammonium sulfide solution (40–48 wt% in H2O, Sigma Aldrich), chloroplatinic acid solution (8 wt% in H2O, Sigma Aldrich), ammonium tetrathiomolybdate (99.97%, Sigma Aldrich), 2-[2-(5-methylfuran-2-yl)vinyl]-4,6-bis(trichloromethyl)-1,3,5-triazine (MFVT, TCI), N-(trifluoromethylsulfonyloxy)-1,8-naphthalimide (IM-NIT, WIMAS Corp.), ethylenediamine (≥99.5%, Sigma Aldrich), 1,2-ethanedithiol (≥98.0%, Sigma Aldrich), anhydrous acetonitrile (99.8%, Sigma Aldrich), anhydrous toluene (99.8%, Sigma Aldrich), N-methyl formamide (99%, Sigma Aldrich), anhydrous dimethyl sulfoxide (≥99.9%, Sigma Aldrich), ethyl alcohol (99.5%, Samchun), and methyl alcohol (99.5%, Samchun) were used in the experiments. All chemicals and reagents were used as received without further purification.
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8

Analytical Reagent Sourcing Protocol

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Brilliant black BN (≥95%), lithol rubine BK (≥95%), sodium phosphate dibasic (≥99.0), citric acid (≥99.5%), and ammonium acetate (≥98%) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Azorubine (95.0%) was purchased from Tokyo Chemical Industry (TCI, Tokyo, Japan). Methanol (≥99.9), acetonitrile (≥99.9), hexane (95%), and acetone (≥99.3) were acquired from J. T. Baker (Philipsburg, NJ, USA). Dimethyl sulfoxide (≥99.0) was purchased from Junsei Chemical (Tokyo, Japan). Ethyl alcohol, anhydrous (≥99.9), and petroleum ether (90.0%) were purchased from Samchun (Seoul, Korea).
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9

Fabrication of ZnO-PAN Composite Membranes

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Polyacrylonitrile (PAN, Mw = 150,000) was purchased from Sigma-Aldrich, Co. (St. Louis, MO, USA). Zinc oxide nanoparticles (ZnO NPs, 99% purity) were supplied by Nanjing XFNANO Materials Tech Co. (Beijing, China). The commercial silane coupling agent, 3-methacryloxypropyltrimethoxysilane (MPTMS, 97% purity), and Methylene Blue (MB, 1% wt/vol aqueous solution) were procured from Alfa Aesar Chemical Co. (Seoul, Korea). Dimethylformamide (DMF, 99.8% purity) and potassium chloride (KCl, >99% purity) were obtained from Daejung Chemicals & Metals Co. (Seoul, Korea). Ethyl alcohol (99.9%, anhydrous) was supplied by Samchun Pure Chemical Co., LTD (Seoul, Korea). All reagents were used as received without further purification.
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10

Synthesis of TiO2-based Nanocomposites

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Tetraethyl orthosilicate (TEOS, 98%) and titanium (IV) isopropoxide (TTIP, 97%) were purchased from Sigma-Aldrich Chemical Co. (Burlington, MA, USA). An ammonia solution (NH4OH, 28.0%), ethyl alcohol (EtOH, 99.9%), acetonitrile (99.8%), sodium borohydride (NaBH4, 98.0%), acetylene carbon black (99.99%), and 250 nm-sized anatase phase titanium dioxide powder (TiO2, 98.5%) were purchased from Samchun Chemical Co. (Seoul, Korea). All the chemicals, reagents, and solvents were used as received without further purification.
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