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CXCR4−/− is a laboratory product used in research applications. It is a cell line that lacks the expression of the CXCR4 protein, which is a chemokine receptor. This cell line can be utilized in studies examining the role and function of the CXCR4 protein.

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Lab products found in correlation

Alexa fluor 488 donkey anti rabbit, by Jackson ImmunoResearch (1 mentions) Alexa fluor 594 donkey anti mouse, by Jackson ImmunoResearch (1 mentions) Ubiquitin, by Merck Group (1 mentions) Dapi nuclear stain, by Thermo Fisher Scientific (1 mentions) Cxcr4, by Abcam (1 mentions) 400 fluorescent microscope, by Nikon (1 mentions) Ubiquitin, by Jackson ImmunoResearch (1 mentions)

2 protocols using cxcr4

1

Timed Pregnancy and Transgenic Mice

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Mice were maintained on a CD-1 background. For timed pregnancies, noon on the day of the vaginal plug was counted as embryonic day 0.5. All mice strains have been previously reported: AI14 Cre-reporter (Madisen et al., 2010 (link)), CXCL-12-GFP (Ara et al., 2003 (link)), CXCR7−/− (Sierro et al., 2007 (link)), CXCR4−/− (Jackson Laboratory 004341), Lhx6-PLAP (Choi et al., 2005 (link)), Lhx6 BAC-GFP (GENSAT), and Nkx2-1-Cre (Xu et al., 2008 (link)). All animal care and procedures were performed according to the University of California at San Francisco Laboratory Animal Research Center guidelines.
Vogt D., Hunt R.F., Mandal S., Sandberg M., Silberberg S.N., Nagasawa T., Yang Z., Baraban S.C, & Rubenstein J.L. (2014). Lhx6 Directly Regulates Arx and CXCR7 to Determine Cortical Interneuron Fate and Laminar Position. Neuron, 82(2), 350-364.
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2

Dual Immunofluorescence Staining of CXCR4 and CXCR7

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Formalin fixed, paraffin embedded tissue slides were double stained for CXCR4 (Abcam Inc., Cambridge MA) and Ubiquitin (Millipore, Temecula CA). A second set of slides was double stained for CXCR7 (Millipore, Temecula CA) and Ubiquitin (Millipore, Temecula, CA. CXCR4 and CXCR7 were detected using the second antibody donkey anti rabbit Alexa Fluor 488 (Jackson Immuno Research Laboratories Inc. West Grove, PA). Ubiquitin was detected using the second antibody donkey anti mouse Alexa Fluor 594 (Jackson Labs. West Grove, PA). All slides were stained with the nuclear stain DAPI (Molecular Probes, Eugene, OR). The slides were examined with a Nikon 400 fluorescent microscope.
Liu H., Li J., Tillman B., Morgan T., French B.A, & French S.W. (2014). TLR3/4 Signaling is Mediated via the NFκB-CXCR4/7 Pathway in Human Alcoholic Hepatitis and Non Alcoholic Steatohepatitis Which Formed Mallory-Denk Bodies. Experimental and molecular pathology, 97(2), 234-240.
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