Cobas integra 400 analyzer

Manufactured by Roche

Sourced in Canada, United States, Switzerland, France

The COBAS INTEGRA 400 analyzer is a clinical chemistry and immunochemistry analyzer designed for professional laboratory use. It is capable of performing a variety of tests on patient samples to aid in medical diagnosis and treatment monitoring. The analyzer utilizes advanced technologies to deliver reliable and accurate results within a specified time frame.

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Lab products found in correlation

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24 protocols using cobas integra 400 analyzer

Metabolic Biomarkers Assessment Protocol

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Glucose concentrations were determined by an automated hexoquinase oxidase system (COBAS INTEGRA 400 analyzer (Roche Diagnostic, Montreal, QC, Canada). Insulin concentrations was determined by an electrochemiluminescence assay (COBAS INTEGRA 400 analyzer (Roche Diagnostic, Montreal, QC, Canada). Insulin resistance was calculated with the formula of the homeostasis model assessment for insulin resistance (HOMA-IR) (glucose x insulin/22.5) [20 (link)]. Different components of the lipid profile (triglycerides, total cholesterol and HDL–cholesterol) were measured using the COBAS INTEGRA 400 analyzer (Roche Diagnostic, Montreal, QC, Canada). LDL cholesterol was obtained in an indirect way through the Friedewald formula (LDL cholesterol = total cholesterol-HDL cholesterol-triglycerides/5) [21 (link)]. C-reactive protein (CRP) was measured by immunoturbimetry (Roche Diagnostics GmbH, Mannheim, Germany).
All adipokines were determined by the Enzyme-Linked Immuno Sorbent Assay (ELISA) as follows: leptin (Diagnostic Systems Laboratories, Inc., Webster, TX, USA) (DSL1023100) [22 (link)], adiponectin (R&D systems, Inc., McKinley, MN, USA) (DRP300) [23 (link)] and resistin (Biovendor Laboratory, Inc., Brno, Czech Republic) (RD191016100) [24 (link)].

de Luis Roman D., Primo D., Izaola O, & Aller R. (2022). Association of the APOA-5 Genetic Variant rs662799 with Metabolic Changes after an Intervention for 9 Months with a Low-Calorie Diet with a Mediterranean Profile. Nutrients, 14(12), 2427.

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Biochemical Blood Analysis Protocol

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After an overnight fasting, whole blood specimens were collected into sodium citrate, heparin and EDTA tubes. Plasma from heparin-containing tubes was immediately used for glycaemia, lipid and high-sensitivity C-Reactive Protein (hsCRP) analyses. Glycaemia was measured using the glucose oxidase method [31 (link)]. Serum levels of total cholesterol, triglycerides and high-density lipoprotein cholesterol were measured by standard enzymatic methods. Analyses were carried out on ARCHITECT c8000 analyzer (Abott Diagnostics, Abbott Park Rd, US) using corresponding reagent kits. The low-density lipoprotein cholesterol was calculated using the formula of Friedewald [32 (link)]. Apolipoprotein A-I andapolipoprotein B 100 levels were measured by turbidmetric immunoassay using COBAS INTEGRA 400 analyzer (Roche Diagnostics Basel, Switzerland). Plasma hsCRP concentrations were measured with a particle-enhanced immune-turbidimetric assay (Roche Tina-Quant CRP, Roche Diagnostics, Basel, Switzerland). Samples of citrate plasma were analyzed within 2 h of venipuncture by an automatic coagulometer (Multifibren, BCS, Siemens Healthcare Diagnostics) for fibrinogen measurements.

Messedi M., Naifar M., Grayaa S., Frikha F., Messoued M., Sethom M.M., Feki M., Kaabach N., Bahloul Z., Jamoussi K, & Ayedi F. (2018). Plasma Saturated and Monounsaturated Fatty Acids in Behçet’s Disease. The Open Rheumatology Journal, 12, 139-151.

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Quantifying Hepatic Enzyme Levels

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Sera samples from uninfected control mice and LM-infected mice were submitted to the University of Texas M.D. Anderson Cancer Center Veterinary Medicine and Surgery Department. Alanine aminotransferase (ALT) was quantitated using an automated COBAS Integra 400 Analyzer (Roche). Data are expressed as mean units per liter ± SEM.

Mueller-Ortiz S.L., Shivshankar P, & Wetsel R.A. (2019). The Second Receptor for C5a, C5aR2, is Detrimental to Mice during Systemic Infection with Listeria monocytogenes. Journal of immunology (Baltimore, Md. : 1950), 203(10), 2701-2711.

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Serum Insulin and Glucose Quantification

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All blood samples were centrifuged and the serum was aliquotted and frozen at −80°C for analysis. Insulin was measured by an electrochemiluminescence immunoassay method using the Elecsys® 2010 Immunoassay System (Roche Diagnostic Systems, Indianapolis, IN, USA). Glucose was assessed by enzymatic colorimetry on the Cobas Integra 400 Analyzer (Roche Diagnostic Systems, Indianapolis, IN, USA).

Dhillon J., Lee J.Y, & Mattes R.D. (2017). The Cephalic Phase Insulin Response to Nutritive and Low-Calorie Sweeteners in Solid and Beverage Form. Physiology & behavior, 181, 100-109.

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Toxicity Evaluation of EO-ZM in Mice

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The toxicity effects of EO-ZM were evaluated by the following procedure; the mice in 3 and 4 groups in comparison with the mice in control group (Group 1) were fasted overnight. The animals were anesthetized by ketamine (100 mg/kg)-xylazine (10 mg/kg) and their thoracic cavity was opened. Blood samples (1.5–2 mL) were collected from the mice heart into cylinders containing ethylenediaminetetraacetic acid (EDTA) anticoagulant and into tubes having no anticoagulant for hematological clinical and chemistry parameters, respectively. Some parameters of hematology such as hemoglobin, hematocrit, white blood cell (WBC), red blood cell (RBC) and platelet counts, were recorded by a Hematological Analyzer, SYSMEX XT-1800i (SYSMEX Co. Japan). Additionally, Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), creatinine (Cr), blood urea nitrogen (BUN), and bilirubin (direct and total) were assessed by COBAS INTEGRA-400 Analyzer (Roche Diagnostics, Germany) on 500 μL of serum samples (17 (link)).

MAHMOUDVAND H., TAVAKOLI KARESHK A., NABI MORADI M., MONZOTE FIDALGO L., MIRBADIE S.R., NIAZI M, & KHATAMI M. (2020). Efficacy and Safety of Zataria multiflora Boiss Essential Oil against Acute Toxoplasmosis in Mice. Iranian Journal of Parasitology, 15(1), 22-30.

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Venous Blood Sampling and Biomarker Analysis

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Venous blood samples were collected using an indwelling cannula inserted in an antecubital vein. Plasma samples for analysis of epinephrine and norepinephrine were collected into tubes containing ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid, and reduced glutathione. Fluoride/oxalate, lithium heparin, and serum tubes were used to collect fasting samples for analysis of glucose, lipids, and insulin, respectively. Serum samples clotted for 1 hour at room temperature (22-24°C) before centrifuging. Plasma samples were centrifuged immediately after collection. All samples were centrifuged at 2000 rpm (931g) for 15 minutes at 4°C and stored at -80°C. Plasma concentrations of epinephrine and norepinephrine were determined from thawed samples by high-performance liquid chromatography with coulometric detection, after extraction from plasma using alumina absorption. 15 (link) Samples were batch analyzed such that all conditions for a given participant were analyzed in the same run by a technician blinded to the study conditions. Fasting glucose was analyzed using the hexokinase method. Fasting insulin was analyzed using a chemiluminescent microparticle immunoassay (Architect ci16200; Abbott Diagnostics, Santa Clara, CA). Fasting lipids were analyzed using a COBAS Integra 400+ analyzer (Roche Diagnostics, Indianapolis, IN).

Wheeler M.J., Dunstan D.W., Ellis K.A., Cerin E., Phillips S., Lambert G., Naylor L.H., Dempsey P.C., Kingwell B.A, & Green D.J. (2019). Effect of Morning Exercise With or Without Breaks in Prolonged Sitting on Blood Pressure in Older Overweight/Obese Adults. Hypertension (Dallas, Tex. : 1979), 73(4).

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Quantification of C1-inhibitor Levels

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To quantify antigenic C1-inhibitor levels, in-house radial immunodiffusion was performed.
The concentration of the functional C1-inhibitor was measured with a C1-inhibitor enzyme immunoassay kit (Quidel, San Diego, CA). The concentration of C1q and C1–INH-Ab were determined by in-house sandwich ELISA methods [23 (link), 24 (link)]. CH50 (total hemolytic activity) of the classical pathway was determined with a hemolytic assay. C3 and C4 concentrations were measured by turbidimetry (Cobas Integra 400 analyzer; Roche, Switzerland).

Polai Z., Balla Z., Varga L., Benedek S, & Farkas H. (2022). Long term follow-up of complement parameters to improve the management of acquired angioedema due to C1-inhibitor deficiency. Heliyon, 8(11), e11292.

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Quantification of Complement Factors

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The following complement parameters were measured right after sampling. To quantify C1-INH concentration, in-house radial immunodiffusion was performed. The level of C1-inhibitor activity was measured with a C1-inhibitor enzyme immunoassay kit (Quidel, San Diego, CA). The concentration of C1q and autoantibodies against C1-INH were determined by in-house sandwich ELISA methods. [38 (link), 39 (link)] CH50 (total hemolytic activity) of the classical pathway was determined with a hemolytic assay. C3 and C4 concentrations were measured by turbidimetry (Cobas Integra 400 analyzer; Roche, Switzerland).

Polai Z., Kajdacsi E., Cervenak L., Balla Z., Benedek S., Varga L, & Farkas H. (2023). C1-inhibitor/C1-inhibitor antibody complexes in acquired angioedema due to C1-inhibitor deficiency. Orphanet Journal of Rare Diseases, 18, 24.

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Comprehensive Blood Biomarker Analysis

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The details of blood sample collection and analysis have already been described (18) . Briefly, in the three study centers, plasma glucose, total cholesterol, HDL cholesterol, and triglyceride concentrations were assayed by enzymatic colorimetric methods (ABX Diagnostics, Montpellier, France; Roche Diagnostic, USA, Indianapolis, IN) on automatic analyzers (ABX Pentra 400, HORIBA Medical, Montpellier, France; COBAS Integra 400 analyzer, Roche Diagnostic Systems, USA, Indianapolis, IN).
Plasma insulin concentrations were measured by ELISA (DIA-source ImmunoAssay S.A., Nivelles, Belgium) on a Triturus analyzer (Diagnostic Grifols S.A., Barcelona Spain) in the Italian center, while they were measured by electrochemiluminescence immunoassay method on the Elecsys 2010 analyzer (Roche Diagnotic Systems USA, Indianapolis, IN) in the Sweden and USA centers. Plasma concentrations of HbA1c were measured by high performance liquid chromatography. LDL cholesterol was calculated using the Friedewald formula. The homeostatic model assessment of insulin resistance (HOMA-IR) was calculated using the following formula: fasting glucose (mg/dL) × fasting insulin (µU/mL)/405 (20) .

Costabile G., Bergia R.E., Vitale M., Hjorth T., Campbell W., Landberg R., Riccardi G, & Giacco R. (2024). Effects on cardiovascular risk factors of a low- vs high-glycemic index Mediterranean diet in high cardiometabolic risk individuals: the MEDGI-Carb study. European journal of clinical nutrition, 78(5).

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Rheumatoid Factor Quantification Protocol

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For rheumatoid factor quantification we used a rheumatoid factor particle-enhanced immunoturbidimetric method (RF II- Tina quant RF II, COBAS) in a COBAS INTEGRA 400 analyzer (Roche Diagnostics). For this, samples were initially diluted (1:5) and then, serial dilutions were prepared automatically (up to 1:128). Samples (50 μl) were incubated with latex particles coated with monoclonal anti-rheumatoid factor antibodies. Agglutination, denoted by formation of aggregates in positive samples, was determined turbidimetrically. Results are expressed as international units (IU) per litre (l) of sample.

Pereira I., Mendes S.J., Pereira D.M., Muniz T.F., Colares V.L., Monteiro C.R., Martins M.M., Grisotto M.A., Monteiro-Neto V., Monteiro S.G., Calixto J.B., Brain S.D, & Fernandes E.S. (2017). Transient Receptor Potential Ankyrin 1 Channel Expression on Peripheral Blood Leukocytes from Rheumatoid Arthritic Patients and Correlation with Pain and Disability. Frontiers in Pharmacology, 8, 53.

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