Rabbit anti ha polyclonal antibody

Rabbit anti-Vps28 monoclonal antibody was purchased from Abcam (Cambridge, MA, USA). Mouse anti-Flag monoclonal antibody and rabbit anti-HA polyclonal antibody were purchased from Proteintech (Wuhan, China). Mouse anti-HA monoclonal antibody was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Mouse anti-actin monoclonal antibody was purchased from CWBIO (Beijing, China). Anti-FMDV structural protein polyclonal pig antiserum was prepared by our laboratory by immunizing pigs with FMDV (O/BY/CHA/2010; GenBank accession no. JN998085.1) VLP. Anti-FMDV nonstructural protein 3A polyclonal rabbit antiserum was prepared by our laboratory by immunizing rabbits with FMDV (O/BY/CHA/2010; GenBank accession no. JN998085.1) nonstructural protein 3A. The secondary antibodies conjugated with horseradish peroxidase (HRP), fluorescein isothiocyanate (FITC), and tetramethyl rhodamine isocyanate (TRITC) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lipofectamine RNAiMAX and Lipofectamine 2000 were purchased from Invitrogen (California, USA). MG132, CQ, Z-VAD-FMK, and CHX were purchased from MedChemExpress (Monmouth Junction, New Jersey, USA). All drugs in this study were dissolved in 0.1% dimethyl sulfoxide (DMSO).

BECN1 siRNAs were synthesized by Thermo Fisher Scientific. The plasmids pCMV-HA-BECN1 and pCMV-HA-USP14 were constructed by ourselves. Antibody against BECN1 was ordered from OriGene (TA502643). Mouse anti-β-actin antibody was purchased from Proteintech (66009–1-lg). Mouse anti-HA monoclonal antibody was ordered from Thermo Fisher Scientific (26183). Rabbit anti-HA polyclonal antibody was ordered from proteintech (51064–2-AP). K48-linkage specific polyubiquitin antibody was purchased from Cell signaling (8081). Mouse and rabbit anti-Vimentin antibodies were ordered from abcam and proteintech (ab8978, 10366–1-AP). The cell cycle-related genes Cyclin B1, CDK1, CDC25C, PCNA were purchased from proteintech (55004–1-AP, 19532–1-AP, 16485–1-AP, 10205–2-AP). The EMT markers E-cadherin, β-catenin, Snail1, and Twist1 were purchased from proteintech (20874–1-AP, 51067–2-AP, 13099–1-AP, 25465–1-AP). Mouse and rabbit anti-USP14 antibodies were ordered from OriGene and proteintech (TA324906, 14517–1-AP). TRITC-conjugated Goat anti-Rabbit IgG and FITC-conjugated Goat anti-mouse IgG were purchased from proteintech (SA00007-2, SA00003-1).

The primary antibodies used for this study were as follows: mouse anti-HBx monoclonal antibody (Santa Cruz, USA), rabbit anti-cGAS monoclonal antibody (CST, USA), rabbit anti-STING monoclonal antibody (CST, USA), rabbit anti-IRF3(Phospho-Ser382) monoclonal antibody (CST, USA), mouse anti-IRF3 monoclonal antibody (CST, USA), mouse anti-β-actin monoclonal antibody (Santa Cruz, USA), mouse anti-GAPDH Monoclonal Antibody(Proteintech, USA), rabbit anti-Flag polyclonal antibody (Proteintech, USA), rabbit anti-HA polyclonal antibody (Proteintech, USA), K48-linked ubiquitin antibody(Billerica, MA, USA), DAPI (Santa Cruz, USA) and Lipofectamine 2000 (Invitrogen, USA) were used following the manufacturer's instructions.