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Tumor necrosis factor α tnf α and interleukin 1β il 1β elisa kits

Manufactured by R&D Systems

The Tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) ELISA kits are laboratory products used to quantitatively measure the levels of these cytokines in biological samples. The kits utilize the enzyme-linked immunosorbent assay (ELISA) technique.

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2 protocols using tumor necrosis factor α tnf α and interleukin 1β il 1β elisa kits

1

Phagocytosis Assay of Neutrophils

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LPS (Escherichia coli serotype 055:B5), formyl methionyl leucyl phenylalanine (fMLP), interleukin-8 (IL-8), phorbol ester (PMA), cell chromatography C (Cytochrome C), superoxide dismutase (SOD), Elastase, Hydroxyethylpiperazine Ethylsulfonic Acid (HEPES) and Emo were obtained from Sigma-Aldrich (St Louis, MO, USA). Tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) ELISA kits were obtained from R&D Systems (Minneapolis, MN). Anti-Myeloperoxidase (MPO) antibody (abcam, ab65871), MNase, RP-1 antibody (BD 550002), SYTOX Green, and Annexin V-FITC were obtained from eBioscience (San Diego, CA). pHrodo red E. coli (Cat.No.4615), pHrodo green S.aureus (Cat. No. 4620) were obtained from Sartorius (Göttingen, Germany). RPMI 1640, fetal bovine serum (FBS), trypsin, and enzyme-free cell dissociation buffer were purchased from Gibco (Grand Island, NY, USA). Penicillin and streptomycin in saline citrate buffer were from Invitrogen (Carlsbad, CA, USA). Other chemical reagents are of analytical grade.
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2

Neutrophil Activation and Dysfunction

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Reagents LPS (Escherichia coli serotype 055:B5), formyl methionyl leucyl phenylalanine (fMLP), interleukin-8 (IL-8), phorbol ester (PMA), cell chromatography C (Cytochrome C), superoxide dismutase (SOD), Elastase, Hydroxyethylpiperazine Ethylsulfonic Acid (HEPES) and Emodin were obtained from Sigma-Aldrich (St Louis, MO, USA). Tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) ELISA kits were obtained from R&D Systems (Minneapolis, MN). Anti-Myeloperoxidase(MPO) antibody(abcam, ab65871), MNase, RP-1 antibody (BD 550002), SYTOX Green, and Annexin V-FITC were obtained from eBioscience (San Diego, CA). pHrodo Red E. coli (Cat.No.4615), pHrodo Green S.aureus (Cat. No. 4620) were obtained from Sartorius (Göttingen, Germany). RPMI 1640, fetal bovine serum (FBS), trypsin, and enzyme-free cell dissociation buffer were purchased from Gibco (Grand Island, NY, USA). Penicillin and streptomycin in saline citrate buffer were from Invitrogen (Carlsbad, CA, USA). Other chemical reagents are of analytical grade.
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