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Cy5 conjugated donkey anti goat igg

Manufactured by Jackson ImmunoResearch
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Cy5-conjugated donkey anti-goat IgG is a secondary antibody that recognizes and binds to goat immunoglobulin G (IgG) molecules. The antibody is conjugated with the fluorescent dye Cy5, which enables detection and visualization of the target goat IgG in various immunoassays and imaging applications.

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Lab products found in correlation

Cy3 conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) C4742 95, by Hamamatsu Photonics (2 mentions) Alexa fluor 488 conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (2 mentions) Hydromount solution, by National Diagnostics (2 mentions) Rmil 6, by R&D Systems (1 mentions) Total stat3, by Cell Signaling Technology (1 mentions) Normal goat igg, by R&D Systems (1 mentions) Stat3 inhibitor stattic, by Santa Cruz Biotechnology (1 mentions) Anti mouse p stat3 tyr705, by Cell Signaling Technology (1 mentions) Infrared dye labeled secondary abs, by LI COR (1 mentions) Mouse monoclonal anti gm130 antibody, by BD (1 mentions) Cy3 conjugated donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions) Rabbit polyclonal anti calnexin, by Santa Cruz Biotechnology (1 mentions) Sheep anti tgn46, by Bio-Rad (1 mentions) Anti ha rat monoclonal antibody, by Roche (1 mentions) Mouse monoclonal anti v5 antibody, by Thermo Fisher Scientific (1 mentions) Rabbit polyclonal anti flag, by Merck Group (1 mentions) Goat polyclonal anti calnexin, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit igg conjugated to horseradish peroxidase, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Donkey anti-goat IgG (29 citations) Cy5TM-conjugated Affini Pure donkey anti-goat IgG (2 citations)

6 protocols using cy5 conjugated donkey anti goat igg

1

Investigating STAT3 Signaling in Macrophages

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Reagents and culture mediums for cell cultures were purchased from MilliporeSigma and Thermo Fisher Scientific. Goat anti-mouse IL-6R polyclonal neutralizing Ab (catalog AF1830) and normal goat IgG (catalog AB-108-C) were purchased from R&D Systems, Bio-Techne. Anti-mouse TLR4/MD2 neutralizing Ab (clone MTS510, catalog 117608) was from BioLegend. STAT3 inhibitor (Stattic) was purchased from Santa Cruz Biotechnology and rmIL-6 was from R&D Systems, Bio-Techne. Abs for flow cytometry were PE anti-mouse p-STAT3 (Tyr705, clone 13A3-1, catalog 651004), PE/Cy7, Pacific blue anti-mouse F4/80 (clone BM8, catalog 123114 and 123124), and PE/Cy7 anti-mouse IL-6R (clone D7715A7, catalog 115814) from BioLegend. Abs for Western blotting included anti-mouse p-STAT3 (Tyr705, catalog 9131) and total STAT3 (catalog 9139) from Cell Signaling Technologies. β-actin Ab (clone AC-15, catalog A5441) from MilliporeSigma. Infrared dye–labeled secondary Abs were from Li-Cor Biosciences. For immunocytochemistry staining and FRET analysis, rabbit anti-mouse CIRP Ab (catalog 10209-2-AP) from ProteinTech was used. Goat anti-mouse CD11b Ab (catalog MBS420973) was from MyBiosource. Fluorescence-labeled secondary Ab Cy3-conjugated donkey anti–rabbit IgG (catalog 711-166-152) and Cy5-conjugated donkey anti–goat IgG (catalog 705-175-147) were from Jackson ImmunoResearch Laboratories.
Zhou M., Aziz M., Denning N.L., Yen H.T., Ma G, & Wang P. (2020). Extracellular CIRP induces macrophage endotoxin tolerance through IL-6R–mediated STAT3 activation. JCI Insight, 5(5), e133715.
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2

Antibody Characterization and Validation

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The following antibodies were used: mouse monoclonal anti-β-actin (cat. no. A1978, 1:10,000; Sigma-Aldrich), rabbit polyclonal anti-FLAG (cat. no. 2368, 1:1000; Cell Signaling), mouse monoclonal anti-PARP-1 (sc8007, 1:1000; Santa Cruz), rabbit polyclonal anti-calnexin (sc11397, 1:1000; Santa Cruz), mouse monoclonal anti-V5 antibody (R960-25, 1:4000; Invitrogen), rat monoclonal anti-HA antibody (12158167001, 1:4000; Roche), rabbit polyclonal anti-HA antibody (71-5500, 1:1000 Invitrogen), goat polyclonal anti-calnexin (sc-6465, 1:200; Santa Cruz), sheep anti-TGN46 (AHP500, 1:200 AbD Serotec) and mouse monoclonal anti-GM130 antibody (610823, 1:200; BD Biosciences). Goat anti-mouse (cat. no. 31430, 1:5,000) and goat anti-rabbit IgG conjugated to horseradish peroxidase (31460, 1:5,000) were from Thermo Fischer Scientific. The following secondary antibodies were used for indirect immuno-fluorescence: FITC-conjugated donkey anti-sheep/goat (STAR88F, 1:200; AbD Serotec), Cy3-conjugated donkey anti-mouse IgG (715-165-150, 1:200; Jackson ImmunoResearch), Cy5-conjugated donkey anti-rabbit IgG (711-175-152, 1:200; Jackson ImmunoResearch), Cy2-conjugated donkey anti-mouse IgG (715-225-150, 1:200; Jackson ImmunoResearch), Cy5-conjugated donkey anti-goat IgG (705-175-147, 1:200; Jackson ImmunoResearch), Cy3-conjugated donkey anti-rabbit IgG (711-165-152, 1:200; Jackson ImmunoResearch).
Cabukusta B., Kol M., Kneller L., Hilderink A., Bickert A., Mina J.G., Korneev S, & Holthuis J.C. (2017). ER residency of the ceramide phosphoethanolamine synthase SMSr relies on homotypic oligomerization mediated by its SAM domain. Scientific Reports, 7, 41290.
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3

Chemogenetic Manipulation of Neuroendocrine Cells

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OxtrCre/+, OxtCre/+, OxtCre/+::OxtrCre/+, CalcaCre/+ and CckCre/+ mice injected with AAV1-DIO-hM3Dq:mCherry, AAV1-DIO-hM4Di:mCherry, control AAV1-DIO-mCherry and AAV-DIO-synaptophysin:mCherry underwent anti-DsRed immunohistochemistry staining to amplify the fluorescent protein signal. To examine neuronal activation, we examined Fos expression in hM3Dq- and control mCherry-injected mice after injection with CNO 2 h before perfusion. The primary antibodies were rabbit anti Ds-Red (1:1,000; (Clontech) Takara Bio USA, number 632496) and goat anti-Fos (1:500, Santa Cruz Biotechnology); the secondary antibodies were Alexa Fluor 594-conjugated donkey anti-rabbit IgG (1:500; Jackson Immunoresearch, number 711-585-152) and Cy5-conjugated donkey anti-goat IgG (1:500; Jackson Immunoresearch, number 705-175-147).
Ryan P.J., Ross S.I., Campos C.A., Derkach V.A, & Palmiter R.D. (2017). Oxytocin-receptor-expressing neurons in the parabrachial nucleus regulate fluid intake. Nature Neuroscience, 20(12), 1722-1733.
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4

Immunofluorescent Staining of Focal Adhesions

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Cells were seeded on 12-mm glass coverslips and fixed for 15 min with 4% paraformaldehyde when they had reached approximately 60-70% confluence, blocked with TBS-containing donkey serum (10%) and incubated overnight at 4°C with the following antibodies diluted in TBS 1X: goat polyclonal anti-Calretinin (1:500; cat#CG1, Swant) and anti-FAK (1:50; Cell Signaling). After washing, the cell-containing coverslips were incubated for 3 h at room temperature with the following secondary antibodies: Alexa Fluor 488-conjugated donkey anti-rabbit IgG (1:100; Jackson Immunoresearch Laboratories, West Grove, PA, USA) and Cy5-conjugated donkey anti-goat IgG (1:100; Jackson Immunoresearch Laboratories). Nuclear DNA was stained using DAPI (5 μg/ml; Molecular Probes, Eugene, OR) and coverslips were mounted with Hydromount solution (National Diagnostics, Atlanta, GA). Images were acquired using a LEICA fluorescent microscope DM6000B (Wetzlar, Germany) equipped with a Hamamatsu camera C4742-95 (Bridgewater, NJ).
Wörthmüller J., Blum W., Pecze L., Salicio V, & Schwaller B. (2018). Calretinin promotes invasiveness and EMT in malignant mesothelioma cells involving the activation of the FAK signaling pathway. Oncotarget, 9(91), 36256-36272.
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5

Immunofluorescence Analysis of Septin 7 and Calretinin

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Cells were seeded on 12-mm glass coverslips and fixed for 15 min with 4% paraformaldehyde. Non-specific binding sites were blocked by incubation with TBS containing donkey serum (10%) for 1 h and coverslips were then incubated overnight at 4 °C with the following antibodies diluted in TBS 1X: goat polyclonal anti-CR (1:500; cat# CG1, Swant, Marly, Switzerland) and rabbit polyclonal anti-septin 7 (1:500; Bethyl Laboratories, USA). After washing, coverslips were incubated with secondary antibodies for 3 h at room temperature with the following secondary antibodies: Alexa Fluor 488-conjugated donkey anti-rabbit IgG (1:100, Jackson Immunoresearch Laboratories, West Grove, PA, USA) and Cy5-conjugated donkey anti-goat IgG (1:100; Jackson). Nuclear DNA was stained using DAPI (5 μg/ml; Molecular Probes, Eugene, OR) and coverslips were mounted with Hydromount solution (National Diagnostics, Atlanta, GA). Images were acquired using a Leica fluorescent microscope DM6000B (Wetzlar, Germany) equipped with a Hamamatsu camera C4742–95 (Bridgewater, NJ). For the cells treated with Bt, cells were seeded onto 12-mm glass coverslips pre-coated with Matrigel (Corning, NY, USA) and treated with 1 mM Bt for 48 h.
Blum W., Pecze L., Rodriguez J.W., Steinauer M, & Schwaller B. (2018). Regulation of calretinin in malignant mesothelioma is mediated by septin 7 binding to the CALB2 promoter. BMC Cancer, 18, 475.
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6

Characterization of CX3CL1 and ADAM10 Interactions

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The primary antibodies used in this study included goat anti-human CX3CL1 immunoglobulin G (IgG) and mouse anti-human ADAM10 IgG from R&D Systems (Minneapolis, MN), rabbit anti-human ADAM10 IgG (Abcam, Toronto, Canada), and rabbit anti-human caveolin-1 IgG (BD Transduction Laboratories, San Jose, CA). All fluorescent and biotin-conjugated secondary antibodies were obtained from Jackson ImmunoResearch Laboratories (Bar Harbor, ME) and include DyLight 549–conjugated donkey anti-goat IgG, Cy5-conjugated donkey anti-goat IgG, DyLight 488–conjugated donkey anti-mouse IgG, DyLight 488–conjugated donkey anti-rabbit IgG, biotin-conjugated rat anti-mouse Fab fragment, and biotin-conjugated rabbit anti-goat Fab fragment. Streptavidin Qdot 655 was obtained from Invitrogen (Burlington, Canada).
The following reagents were used: MβCD, Lat B, and Toxin B from Sigma-Aldrich (Oakville, Canada), Cal A (Calbiochem, Gibbstown, NJ), Draq5 (Biostatus, Shepshed, United Kingdom), fibronectin (Roche, Mississauga, Canada), filipin from Streptomyces filipinensis (Polysciences, Warrington, PA), wheat germ agglutinin (WGA; Life Technologies), TAPI-2 (Peptides International, Louisville, KY), and G1254023X (Tocris, Minneapolis, MN).
Wong H.S., Jaumouillé V., Heit B., Doodnauth S.A., Patel S., Huang Y.W., Grinstein S, & Robinson L.A. (2014). Cytoskeletal confinement of CX3CL1 limits its susceptibility to proteolytic cleavage by ADAM10. Molecular Biology of the Cell, 25(24), 3884-3899.
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