Real envision hrp rabbit mouse polymer

For immunohistochemistry, heat‐induced epitope retrieval was performed with citrate buffer. Staining was performed on a DAKO autostainer plus. After blocking endogenous peroxidase, sections were incubated for 45 minutes with rabbit polyclonal Anti‐TLR7 antibody (1:50; Proteintech #17232‐1‐AP) or anti‐CD68 antibody (1:100; Dako #MO876), respectively. Sections were washed and incubated with Dako REAL EnVision HRP Rabbit/Mouse polymer, which reacts with DAB‐Chromogen, according to the manufacturer's protocol. Histology and staining results were evaluated in a blinded fashion by an experienced pathologist (C. Keber). Only cases where at least 2 independent biopsy cores could be evaluated were counted. Staining intensity was graded on a scale from 0 to 3 (absent, weak, moderate, strong), and a mean score was calculated for each patient.

For immunohistochemistry, heat-induced epitope retrieval was performed with EDTA. Staining was performed on a DAKO autostainer plus. After blocking endogene peroxidase, sections were incubated for 45 min with mouse monoclonal anti-VEGFA antibody (1:200; Abcam no. ab1316, clone VG-1). Sections were washed and incubated with Dako REAL EnVision HRP Rabbit/Mouse polymer, which reacts with DAB chromogen, according to the manufacturer’s protocol.