Sw620

HepG2, MNK-45, MCF-7, SW620, and A549 cell lines were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). SMMC-7721 cell line was purchased from BeNa Culture Collection (Beijing, China). MNK-45, SMMC-7721, and SW620 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Solarbio, Beijing, China); andHepG2, MCF-7,and A549 cells were cultured in Dulbecco’s modified eagle’s medium (DMEM) with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Solarbio, Beijing, China).All cells were incubated at 37 °C in a humidified 5% CO₂ atmosphere.

Colon cancer HT-29 (catalog numbers: CL-0118), HCT-116 (catalog numbers: CL-0096), SW480 (catalog numbers: CL-0223), and SW620 (catalog numbers: CL-0225B) cells were purchased from Procell (Wuhan, China), and human immortalized colon cells (NCM460, catalog numbers: BNCC339288) were purchased from BNCC (Beijing, China). HT-29 and HCT-116 cell lines were cultured in RPMI‐1640 medium (catalog numbers: 31800, Solarbio, Beijing, China). SW480, SW620, and NCM460 cells were cultured in DMEM culture media (catalog numbers: 12100, Solarbio, Beijing, China). All cell culture media were supplemented with penicillin G (100 μg/mL), streptomycin (100 μg/mL), and 10% fetal bovine serum (FBS; catalog numbers: 164210-50, Wuhan, China), and the cells were grown at 37°C with 5% CO2. The logarithmic growth cells were taken for the experiment. We used Lipofectamine 3000 (Thermo Fisher; catalog numbers: L3000015; Shanghai, China) and GSTO2 siRNA (GenePharma, Shanghai, China; siRNA#1 ID113395, siRNA#2 ID 113396, siRNA#3 ID113397) to make transfections in colon cancer cells based on the provided directions. Western blot (WB) and qRT-PCR were used to detect cell transfection efficiency.