J 820 spectropolarimeter

Optical rotation values were determined using a JASCO P-1020 polarimeter. UV spectra were recorded using a Hitachi U-2900 spectrometer. ECD spectra were acquired with a JASCO J-820 spectropolarimeter and IR spectra were recorded using a Shimadzu FTIR-8400S spectrophotometer. NMR spectra were acquired with a JEOL JNM-ECZ 400S spectrometer with tetramethylsilane as an internal standard. ESI–MS data were obtained using an Agilent 6230 LC/TOF mass spectrometer. HPLC was performed on a Hitachi HPLC system equipped with an L-2130 pump, an L-2200 autosampler, an L-2300 column oven, and an L-2455 diode array detector. Silica gel AP-300 (Toyota Kako), Sephadex LH-20 (GE Healthcare), and Cosmosil 75C₁₈-OPN (Nacalai Tesque) were used for column chromatography (CC). Silica gel 60 F₂₅₄ and RP-18 F_254S (Merck) were used for TLC analysis.

Optical rotation values were recorded on a JASCO P-2200 polarimeter, while the UV spectra were obtained using a Hitachi U-2900 spectrophotometer. The ECD spectra were acquired on a JASCO J-820 spectropolarimeter and the IR spectra were recorded on a Shimadzu FTIR-8400S spectrophotometer. NMR spectra were acquired on a JEOL JNM-ECZ 400S spectrometer with tetramethylsilane as the internal standard. ESIMS data were obtained using an Agilent 6230B TOF LC/MS system equipped with an electrospray ion source (Agilent Technologies, CA, USA) and an Agilent 1260 infinity II LC (Agilent Technologies). DNA sequencing was performed using an Applied Biosystems 3130 genetic analyzer. Silica gel AP-300 (Toyota Kako) was employed for column chromatography (CC). Silica gel 60 F₂₅₄ and RP-18 F_254S (both Merck) were used for TLC.