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Barnstead nanopure infinity water purification system

Manufactured by Thermo Fisher Scientific
Sourced in United States

The Barnstead Nanopure Infinity water purification system is a laboratory-grade water purification device designed to produce high-purity water. It utilizes a multi-stage purification process to remove impurities and contaminants from feed water, delivering purified water that meets the requirements for various laboratory applications.

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3 protocols using barnstead nanopure infinity water purification system

1

Antimicrobial Chitosan Nanoparticle Synthesis

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Low molecular weight chitosan (LMW CS, poly (d-glucosamine), Mw = 460,000 Da), low molecular weight polydiallyldimethylammonium chloride (LMW pDADMAC, 20 wt % in water, Mw = 100–200 Da), poly (acrylic acid) (PAA, 35 wt % in water, Mw = 250,000 Da), glycerol (1,2,3-propanetriol glycerin, ≥99%), propium iodide solution (PI, 1.0 mg/mL in water), diiodomethane (≥99.0%), Mueller Hinton broth (MHB), and ReagentPlus® grade acetic acid (AA, ≥99.0%) were obtained from Sigma-Aldrich (St. Louis, MO, USA). SYTO® 9 green fluorescent nucleic acid stain (S34854, 5 mM solution in dimethyl sulfoxide) was purchased from Life Technologies (Grand Island, NY, USA). Sodium hydroxide (NaOH) was purchased from Fisher Scientific (Fair Lawn, NJ, USA). Difco Luria-Bertani (LB) broth was purchased from BD Life Sciences (Franklin Lakes, NJ, USA). Deionized (DI) water was obtained from a Barnstead Nanopure Infinity water purification system (Thermo Fisher Scientific, Waltham, MA, USA).
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2

Conductive PEDOT:PSS Polymer Films

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All chemicals were used as
received. Poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS)
was purchased from Sigma-Aldrich as a dispersion in water with 1.3
wt % solid content (0.5 wt % PEDOT and 0.8 wt % PSS) and a reported
conductivity of 1 S/cm. Acetic acid, glutaraldehyde solution (50 wt
% in water), and Mowial 18-88 poly(vinyl alcohol) (PVA) with a molecular
weight of 130 kDa and degree of hydrolysis of 87–89% were also
purchased from Sigma-Aldrich. Sodium hydroxide (NaOH), acetone, Sparkleen
1, sodium iodide (NaI), sodium bromide (NaBr), sodium chloride (NaCl),
and magnesium chloride (MgCl2) were purchased from Fisher
Scientific. Deionized (DI) water was obtained from a Barnstead Nanopure
Infinity water purification system (Thermo Fisher Scientific, Waltham,
MA).
Fisherbrand microscope glass slides were purchased from
Fisher Scientific. Interdigitated electrodes (IDEs) consisted of 20
platinum electrodes (10 pairs) that were 50 nm thick and 4.5 mm in
length, with a 100 μm spacing (Figure S1A). The IDEs were fabricated in Dr. Jun Yao’s Laboratory (Department
of Electrical and Computer Engineering, UMass Amherst) on Si wafers
(N-Type, P doped, single-side polished with 1 μm thermal oxide
layer purchased from University Wafer, Boston, MA). Then, a 30-gauge
(0.31 mm) copper wire was attached to each electrode by using silver
conductive epoxy adhesive (MG Chemicals).
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3

Synthesis and Characterization of PEG-Dopamine Conjugates

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All materials were used as received unless otherwise stated. Dopamine hydrochloride (dopamine), succinimidyl-carboxymethyl-ester-terminated poly(ethylene glycol) (PEG, >95%, Mn = 40 kDa, PDI <1.10), bovine serum albumin (BSA, 66 kDa, heat shock fraction, >98%), Bradford reagent (1−1400 μg/mL), phosphate-buffered saline (PBS, 1× sterile biograde), Luria−Bertani broth (LB), sodium chloride (NaCl), M9 minimal salts (M9 media), D-(+)-glucose, and calcium chloride (anhydrous) were purchased from Sigma-Aldrich (St. Louis, MO). Tris-(hydroxymethyl)aminomethane (tris), acetone (histological grade), sodium hydroxide (NaOH), ethanol (absolute anhydrous), and isopropyl alcohol (IPA, Certified ACS Plus) were purchased from Fisher Scientific (Pittsburgh, PA). Poly(2-methacryloyloxyethyl phosphorylcholine) (polyMPC, Mn = 30 kDa) was prepared according to a previously published method.47 (link) Hydroxy-terminated PEG (Mn = 4, 55, 95, 130, and 203 kDa) was purchased from Polymer Source (Quebec, Canada). Deionized (DI) water was obtained from a Barnstead Nanopure Infinity water purification system (Thermo Fisher Scientific, Waltham, MA).
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