Biotin streptavidin horseradish peroxidase system

Manufactured by GE Healthcare

Sourced in United Kingdom

The Biotin/streptavidin-horseradish peroxidase system is a laboratory tool used in various biological and biochemical applications. It facilitates the detection and visualization of target molecules through the interaction between biotin and streptavidin, which is coupled with the enzymatic activity of horseradish peroxidase.

Automatically generated - may contain errors

Lab products found in correlation

Renaissance western blot chemoluminescence reagent, by PerkinElmer (4 mentions) Ps6 ser235 236, by Cell Signaling Technology (1 mentions) Anti gapdh ab8245, by Abcam (1 mentions) Ab15348, by Abcam (1 mentions) Ab109131, by Abcam (1 mentions) Ab8245, by Abcam (1 mentions) Chemostar imager, by Intas (1 mentions) Anti bcl 2, by BD (1 mentions)

4 protocols using biotin streptavidin horseradish peroxidase system

Western Blot Analysis of Cell Signaling

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were prepared as described previously^{40 (link)}. Anti AKT (#4691), pAKT (Ser473) (#4060), BAD (#9239), pBAD (Ser136) (#4366), BCL2 (#4223), BCL2A1 (#14093), BCL-W (#2724), BCLXL (#2764), pGSK3B (Ser9) (#9323), MCL1 (#39224), pMCL1 (Ser159/Thr163) (#4579), S6 (#2317) and pS6 (Ser235/236) (#2211) antibodies were purchased from Cell Signaling (New England Biolabs, Frankfurt, Germany). Anti GAPDH (ab8245) antibody was from Abcam (Cambridge, UK). Specific bands on nitrocellulose membranes were visualized with the biotin/streptavidin–horseradish peroxidase system (GE Healthcare, Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” protocol (Perkin Elmer, Waltham, MA, USA).

Quentmeier H., Geffers R., Hauer V., Nagel S., Pommerenke C., Uphoff C.C., Zaborski M, & Drexler H.G. (2022). Inhibition of MCL1 induces apoptosis in anaplastic large cell lymphoma and in primary effusion lymphoma. Scientific Reports, 12, 1085.

+ Open protocol

+ Expand

Western Blot Protein Detection

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were prepared as described previously^{75 (link)}. Anti BLIMP1 (#9115), CCND2 (#3741S), S100A4 (#13018S), S100A6 (#13162S) Abs were purchased from Cell Signaling (New England Biolabs, Frankfurt, Germany). Anti GAPDH (ab8245) and anti S100A2 (ab 109494) Abs were from Abcam (Cambridge, UK). Specific bands on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare, Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” protocol (Perkin Elmer, Waltham, MA, USA).

Quentmeier H., Pommerenke C., Dirks W.G., Eberth S., Koeppel M., MacLeod R.A., Nagel S., Steube K., Uphoff C.C, & Drexler H.G. (2019). The LL-100 panel: 100 cell lines for blood cancer studies. Scientific Reports, 9, 8218.

+ Open protocol

+ Expand

Western Blot for ACE2, TMPRSS2, and GAPDH

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies against ACE2 (ab15348), TMPRSS2 (ab109131), and GAPDH (ab8245) were obtained from Abcam (Cambridge, UK). Western blot samples were prepared as described previously [66 (link)]. Proteins on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare; Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” (Perkin Elmer; Waltham, MA, USA). Documentation was performed using the digital system ChemoStar Imager (INTAS, Göttingen, Germany).

Pommerenke C., Rand U., Uphoff C.C., Nagel S., Zaborski M., Hauer V., Kaufmann M., Meyer C., Denkmann S.A., Riese P., Eschke K., Kim Y., Safranko Z.M., Kurolt I.C., Markotic A., Cicin-Sain L, & Steenpass L. (2021). Identification of cell lines CL-14, CL-40 and CAL-51 as suitable models for SARS-CoV-2 infection studies. PLoS ONE, 16(8), e0255622.

+ Open protocol

+ Expand

Western Blot Analysis of Apoptosis Markers

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were prepared as described previously [26 (link)]. Anti BCL2 (1:500 dilution) and anti MCL1 Abs (1:250) were purchased from Becton Dickinson. Abs against the phosphorylated and unphosphorylated forms of p38 and MAP2K3 (1:10,000 each) and against BCL6 (1:1,000), BCLXL (1:1,000) and MYC (1:2,000) were obtained from Cell Signaling Technology (Leiden, The Netherlands), the GAPDH mouse monoclonal Ab (mAb) (1:5,000) was from Abcam (Cambridge, UK). Specific bands on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare, Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” protocol (Perkin Elmer, Waltham, MA, USA).

Quentmeier H., Drexler H.G., Hauer V., MacLeod R.A., Pommerenke C., Uphoff C.C., Zaborski M., Berglund M., Enblad G, & Amini R.M. (2016). Diffuse Large B Cell Lymphoma Cell Line U-2946: Model for MCL1 Inhibitor Testing. PLoS ONE, 11(12), e0167599.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!