Phospho p65 3033

Manufactured by Cell Signaling Technology

Sourced in United States

Phospho-p65 (#3033) is a primary antibody that detects endogenous levels of p65/RelA only when phosphorylated at serine 536. It can be used for Western blotting applications.

Automatically generated - may contain errors

Lab products found in correlation

Cdk4 sc 260, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Phospho-p65 (148 citations) Anti-phospho-P65--NF-κB (#3033s), (2 citations)

2 protocols using phospho p65 3033

Whole-cell Protein Extraction and Analysis

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Whole-cell lysates were extracted in RIPA extraction buffer, and protein concentrations were determined using a BCA protein assay (Pierce; Thermo Fisher Scientific, Inc., Waltham, MA, USA). Cell lysates (30 μg) were mixed with 20 μL of SDS-PAGE sample buffer and loaded on 4–12% SDS-PAGE gels. Antibodies against HECTD4 (MBP1-31,944) were obtained from Novus Biological (Centennial, CO, USA). Antibodies against β-actin (#8457), phospho-JNK (#4668), phospho-Ikb (#9246), and phospho-p65 (#3033) were purchased from Cell Signaling Technology (Beverly, MA, USA).

Lee Y.J., Lee H., Jang H.B., Yoo M.G., Im S., Koo S.K, & Lee H.J. (2022). The potential effects of HECTD4 variants on fasting glucose and triglyceride levels in relation to prevalence of type 2 diabetes based on alcohol intake. Archives of Toxicology, 96(9), 2487-2499.

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Comprehensive Protein Expression Analysis

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Protein expression levels were analyzed by standard Western blotting protocols. The following antibodies were used in this study: hBD3 antibody (500-P241) from PeproTech (Rocky Hill, NJ, USA); AKT (pan) (#4691), ERK 1/2 (#9102), phospho-AKT (#2965), phospho-ERK1/2 (#4376), p65 (#8242) and phospho-p65 (#3033) antibodies from Cell Signaling Technology (Danvers, MA, USA). Cyclin A (sc-596), cyclin E (sc-247), Cdk2 (sc-163) and Cdk4 (sc-260) from Santa Cruz Biotechnology (Dallas, TX, USA); β-actin (SP124) antibody from Sigma-Aldrich (USA). Cyclin D1/D2 was from R&D Systems (Minneapolis, MN, USA).

Xu D., Zhang B., Liao C., Zhang W., Wang W., Chang Y, & Shao Y. (2016). Human beta-defensin 3 contributes to the carcinogenesis of cervical cancer via activation of NF-κB signaling. Oncotarget, 7(46), 75902-75913.

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