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603 1.49 na tirf objective

Manufactured by Oxford Instruments

The 603 1.49 NA TIRF objective is a high-numerical aperture (NA) objective lens designed for total internal reflection fluorescence (TIRF) microscopy. It provides a high angle of illumination necessary for TIRF imaging. The objective has a numerical aperture of 1.49, which allows for the efficient collection of fluorescence signals. This product is suitable for a range of TIRF applications in life science research.

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2 protocols using 603 1.49 na tirf objective

1

Live-cell Imaging of Cellular Dynamics

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Cells were imaged using a Nikon TE-2000E inverted microscope with a 603 1.49 NA TIRF objective, Andor Revolution XD spinning disk confocal system, and 488 and 568 nm solid-state lasers. Cells were imaged in Opti-MEM or Leibowitz's L15 medium (Gibco), 5% FBS, at 37°C. Time lapses were acquired using an Andor iXon+ EM-CCD camera using Andor IQ. Original 16-bit tif files acquired directly from the camera were used for image analysis.
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2

Live-cell Imaging of Cellular Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were imaged using a Nikon TE-2000E inverted microscope with a 603 1.49 NA TIRF objective, Andor Revolution XD spinning disk confocal system, and 488 and 568 nm solid-state lasers. Cells were imaged in Opti-MEM or Leibowitz's L15 medium (Gibco), 5% FBS, at 37°C. Time lapses were acquired using an Andor iXon+ EM-CCD camera using Andor IQ. Original 16-bit tif files acquired directly from the camera were used for image analysis.
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