Staphylococcus enterotoxin B (SEB) was purchased from Toxin Technologies (Sarasota, FL, USA). Delta‐9‐Tetrahydrocannabinol (THC) was procured from (NIH National Institute on Drug Abuse, National Institutes of Health, Bethesda, MD, USA). The following reagents (culture medium reagents: RPMI 1640, L‐Glutamine, Penicillin‐Streptomycin, HEPES, FBS, and PBS) were purchased from Invitrogen Life Technologies (Carlsbad, CA). We purchased the following antibodies and Fix/Perm buffer from Biolegend (San Diego, CA, USA). Fc Block reagent was purchased from BD Pharmingen (Carlsbad, CA). True‐Nuclear™ Transcription Factor Buffer Set was purchased from BioLegend. RNeasy and miRNAeasy Mini kits, miScript primer assays kit, and miScript SYBR Green PCR kit were purchased from QIAGEN (QIAGEN, Valencia, CA). iScript and miScript cDNA synthesis kits were purchased from Bio‐Rad (Madison, WI) and Epicentre’s PCR premix F and Platinum Taq DNA Polymerase kits were purchased from Invitrogen Life Technologies (Carlsbad, CA). Ficoll‐Histopaque®‐1077 (Sigma‐Aldrich) ELISA kits for (ELISA MAXTM Standard SET Mouse) were purchased from Biolegend. Immunofluorescence antibodies were purchased from Abcam (Cambridge, MA) PBS twice.
Epicentre s pcr premix f
Manufactured by Thermo Fisher Scientific
Sourced in United States
Epicentre's PCR premix F is a ready-to-use solution for performing polymerase chain reaction (PCR) experiments. It contains all the necessary components, including buffer, dNTPs, and a thermostable DNA polymerase, pre-mixed and optimized for efficient PCR amplification.
Automatically generated - may contain errors
Lab products found in correlation
L glutamine, by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Hepes, by Thermo Fisher Scientific (3 mentions)
Miscript primer assays kit, by Qiagen (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (3 mentions)
Miscript sybr green pcr kit, by Qiagen (3 mentions)
Rneasy and mirnaeasy mini kits, by Qiagen (2 mentions)
Platinum taq dna polymerase kit, by Thermo Fisher Scientific (2 mentions)
Platinum taq polymerase, by Thermo Fisher Scientific (2 mentions)
Ficoll histopaque 1077, by Merck Group (1 mentions)
Fix perm buffer, by BioLegend (1 mentions)
True nuclear transcription factor buffer set, by BioLegend (1 mentions)
Fc block reagent, by BD (1 mentions)
Iscript and miscript cdna synthesis kits, by Bio-Rad (1 mentions)
Miscript cdna synthesis kit, by Qiagen (1 mentions)
Acridine orange solution, by Merck Group (1 mentions)
Epitect bisulfite kit, by Qiagen (1 mentions)
Mirneasy kit, by Qiagen (1 mentions)
4 protocols using epicentre s pcr premix f
1
Multifaceted Immunological Assays Protocol
2
Diethylstilbestrol and Acridine Orange Protocol
3
Immune Modulation Protocol using miR-185-5p
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The following chemicals were purchased and used: SEB (Toxin Technologies, Sarasota, FL, United States), RES and DMSO (Sigma-Aldrich, St. Louis, MO, United States), culture medium (RPMI 1640), Penicillin/Streptomycin, HEPES, L -glutamine, FBS, and PBS (Invitrogen Life Technologies, Carlsbad, CA, United States). Fluorophore-labeled anti-mouse CD3, CD4, CD8, CD44, NK1.1, CD11b, and Gr1 antibodies were purchased from eBioScience (Carlsbad, CA, United States). Bio-Plex kit for mouse cytokines was purchased from Bio-Rad (Bio-Rad, Hercules, CA, United States). Polymerase chain reaction (PCR) reagents, Epicentre’s PCR premix F and Platinum Taq Polymerase, were purchased form Invitrogen Life Technologies (Carlsbad, CA, United States). miRNeasy kit, miScript cDNA synthesis kit, miScript primer assays kit, miScript SYBR Green PCR kit, miR-185-5P mimic, and miR-185-5p inhibitor, SsoAdvanced SYBR green supermix from Bio-Rad (Hercules, CA, United States) were purchased from QIAGEN (Qiagen, Inc., Valencia, CA, United States).
4
Immunomodulatory Effects of THC and SEB
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Staphylococcus enterotoxin B (SEB) was purchased from Toxin Technologies (Sarasota, FL, USA). Delta-9-Tetrahydrocannabinol (THC) was procured from the National Institute on Drug Abuse at the National Institutes of Health (Bethesda, MD, USA). RPMI 1640 culture medium, L-glutamine, penicillin-streptomycin mixture, HEPES buffer, fetal bovine serum, and phosphate buffered saline (PBS) were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). RNeasy and miRNAeasy Mini kits, miScript primer assays kit, and miScript SYBR Green PCR kits were purchased from QIAGEN (Valencia, CA, USA). The iScript and miScript cDNA synthesis kits were purchased from Bio-Rad (Madison, WI, USA). Epicentre’s PCR premix F and Platinum Taq DNA Polymerase kits were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA).
ELISA kits for IL-2, IL1β, CCL2, and CCL5 (ELISA MAXTM Standard SET Mouse) were purchased from Biolegend. Seahorse XFp Glycolytic Rate Assay Kits and XFP Cell Mito Stress Test Kits were purchased from Agilent Technologies (Santa Clara, CA, USA).
ELISA kits for IL-2, IL1β, CCL2, and CCL5 (ELISA MAXTM Standard SET Mouse) were purchased from Biolegend. Seahorse XFp Glycolytic Rate Assay Kits and XFP Cell Mito Stress Test Kits were purchased from Agilent Technologies (Santa Clara, CA, USA).
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