A0021

Blood samples were drawn at admission, before surgery. The sera were analyzed for routine chemical parameters, including Ca, albumin and for DBP, t-25OHD, PTHi. All measurements were carried out in a fully automated manner in different runs. t-25OHD was measured by a competitive electro-chemiluminescence (ECL) protein binding assay—inter-assay coefficients of variance (CV%): 6.2–9.7%, at decreasing concentrations in the range of 50.0–16.7 nmol/L and limit of detection: 9.8 nmol/L. The PTHi was assessed by a sandwich principle using ECL techniques—inter-assay CV% were 2.6–6.5%, at the dropping concentration range of 676–27 pg/mL (71.5–2.9 pmol/L). The functional sensitivity: 6 pg/mL (0.64 pmol/L) with the same system as t-25OHD was measured (Cobas e411 analyser, Roche, Rotkreuz, Switzerland). Estimations of the DBP levels were carried out by an immunoturbidometric assay using polyclonal rabbit anti human Gc-globulin antibody (A0021, Dako, Glostrup, Denmark), configured for the Modular System (Roche, Mannheim, Germany). The measuring range of DBP method was 25–495 mg/L, the detection limit was estimated to be 7.6 mg/L. The total CVs were 3.5–6.1% at 405–95 mg/L concentrations. Albumin and Ca levels were determined by a colorimetric assay with endpoint methods using Modular analyser (Roche). The inter-assay CV% exceeded 2% in none of the assays.