Stained sections were viewed with a Leica DM750 Digital Light microscope, and digital photomicrographs were taken by an attached Leica ICC50 camera. Image Analysis and Processing for Java (ImageJ), a public domain software sponsored by the National Institute of Health (USA), was used to analyze and quantify the photomicrographs. The immunohistochemical stained sections were analyzed for GFAP reactivity using the ImmunoRatio plugin.
Dm750 digital light microscope
Manufactured by Leica
The Leica DM750 Digital Light Microscope is a high-quality optical instrument designed for various laboratory applications. It features a digital camera that captures images and video, allowing for digital documentation and analysis of specimens. The microscope provides clear, detailed images with its high-resolution optics and illumination system. Its core function is to magnify and observe small samples for scientific, educational, or industrial purposes.
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Lab products found in correlation
2 protocols using dm750 digital light microscope
1
Quantifying GFAP Reactivity in Tissue
2
Quantifying Neurodegeneration in Microscopy
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Stained sections were observed under a Leica DM750 digital light microscope and digital photomicrographs obtained via attached Leica ICC50 camera. H&E stained photomicrographs were imported into Image J software (NIH-sponsored public domain image analysis software). Neurons showing degenerating features were noted and counted using the Image J cell counter tool.
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