In the examining Institute for Medical Diagnostics, Nicolaistrasse 22, 12247 Berlin inspected by DAKKS (Deutsche Akkreditierungsstelle GmbH, accredited to DIN EN ISO/IEC 17025:2005 and DIN EN ISO 15189:2007), the samples were homogenized by mechanical force in 200 μL of cold protease inhibitor buffer (Complete Mini Protease Inhibitor Cocktail; Roche Diagnostics GmbH, Penzberg, Germany). The homogenate was then centrifuged for 15 minutes at 13,400 rpm. Next, the supernatant was collected and centrifuged for further 25 minutes at 13,400 rpm. In the 15 supernatants of tissue homogenate, we measured, regulated on activation, normal T-cell expressed and secreted (RANTES), also known as chemokine C-C motif ligand 5 (CCL5), FGF-2, interleukin- (IL-) 1 receptor antagonist (ra), IL-6, IL-8, monocyte chemotactic protein-1 (MCP1), and tumor necrosis factor-alpha (TNF-α). Measurement was performed using the Human Cytokine/Chemokine Panel I (MPXHCYTO-60K; Merck KGaA, Darmstadt, Germany) according to the manufacturer's instructions and analyzed using Luminex 200 with xPonent software (Luminex Co, Austin, TX, USA).
Human cytokine chemokine panel 1 mpxhcyto 60k
Manufactured by Merck Group
Sourced in Germany
The Human Cytokine/Chemokine Panel I (MPXHCYTO-60K) is a multiplex assay used for the quantitative detection of various human cytokines and chemokines in biological samples. The panel includes a pre-configured set of analytes that can be measured simultaneously in a single well. This lab equipment is designed to provide researchers with a comprehensive tool for profiling the human immune response and inflammatory status.
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Lab products found in correlation
5 protocols using human cytokine chemokine panel 1 mpxhcyto 60k
1
Quantifying Tissue Cytokine Profiles
2
Serum RANTES Levels in Multiple Sclerosis
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Serum values were collected from 11 participants to evaluate the serum R/C levels (S-R/C) as a parameter of MS. S-R/C levels were measured before BMDJ/FDOJ surgery. The serum sample was taken intravenously from all patients using a heparinized 7.5 mL collection tube (Sarstedt, Newton, NC, USA). The measurement of S-R/C in serum was performed using the Human Cytokine/Chemokine Panel I (MPXHCYTO-60K; Merck KGaA, Darmstadt, Germany) according to the manufacturer’s instructions and analyzed using the Luminex® 200TM with xPonent® Software (Luminex Co). To measure the serum RANTES levels, the samples were prediluted to 1:100 in sample buffer according to manufacturer’s instructions (The Examining Institute for Medical Diagnostics, Berlin, Germany; inspected by DAKKS [Deutsche Akkreditierungsstelle GmbH; accredited to DIN EN ISO/IEC 17025:2005 and DIN EN ISO 15189:2007]).
3
Quantification of Inflammatory Mediators
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At the examining Institute for Medical Diagnostics, Nikolaistr. 22, D-12247 Berlin (inspected by DAKKS [Deutsche Akkreditierungsstelle GmbH; accredited to DIN EN ISO/IEC 17025:2005 and DIN EN ISO 15189:2007]), the samples were homogenized by mechanical force in 200 μL of cold protease inhibitor buffer (Complete Mini Protease Inhibitor Cocktail; Roche Diagnostics GmbH, Penzberg, Germany). The homogenate was centrifuged for 15 min at 13,400 rpm. Afterwards, the supernatant was collected and centrifuged for a further 25 min at 13,400 rpm. In the 15 supernatants of tissue homogenate, we measured regulated on activation, normal T-cell expressed and secreted (RANTES), fibroblast growth factor (FGF)-2, interleukin (IL)-1 receptor antagonist (ra), IL-6, IL-8, monocyte chemotactic protein-1 (MCP1), and tumor necrosis factor-alpha (TNF-α). Measurement was performed using the Human Cytokine/Chemokine Panel I (MPXHCYTO-60K; Merck KGaA, Darmstadt, Germany) according to the manufacturer’s instructions, and these findings were analyzed using the Luminex® 200™ with xPonent® Software (Luminex Co, Austin, TX, USA).
4
Cytokine Profiling of Osteoimmune Samples
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Samples with conspicuous osteoimmunological abnormalities that were excised during surgery were cooled and analyzed in the laboratory to determine their individual cytokine profiles (Figure 5 ). At IMD-Berlin (inspected by Deutsche Akkreditierungsstelle, the national accreditation body of the Federal Republic of Germany34
), the samples were homogenized by mechanical force in 200 μL of cold protease inhibitor buffer (Complete Mini Protease Inhibitor Cocktail; Roche Diagnostics GmbH, Penzberg, Germany). The homogenate was then centrifuged for 15 minutes at 13,400 rpm. Next, the supernatant was collected and centrifuged for a further 25 minutes at 13,400 rpm. In the 15 supernatants of tissue homogenate, we measured CCL5, fibroblast growth factor (FGF)-2, IL-1 receptor antagonist (ra), IL-6, IL-8, monocyte chemotactic protein-1 (MCP1), and TNF-α. These measurements were performed using the Human Cytokine/Chemokine Panel I (MPXHCYTO-60K; Merck KGaA, Darmstadt, Germany) according to the manufacturer’s instructions. The findings were analyzed using the Luminex® 200™ with xPonent® Software (Luminex Co, Austin, TX, USA).
), the samples were homogenized by mechanical force in 200 μL of cold protease inhibitor buffer (Complete Mini Protease Inhibitor Cocktail; Roche Diagnostics GmbH, Penzberg, Germany). The homogenate was then centrifuged for 15 minutes at 13,400 rpm. Next, the supernatant was collected and centrifuged for a further 25 minutes at 13,400 rpm. In the 15 supernatants of tissue homogenate, we measured CCL5, fibroblast growth factor (FGF)-2, IL-1 receptor antagonist (ra), IL-6, IL-8, monocyte chemotactic protein-1 (MCP1), and TNF-α. These measurements were performed using the Human Cytokine/Chemokine Panel I (MPXHCYTO-60K; Merck KGaA, Darmstadt, Germany) according to the manufacturer’s instructions. The findings were analyzed using the Luminex® 200™ with xPonent® Software (Luminex Co, Austin, TX, USA).
5
Cytokine Profiling in Maxillofacial Tissues
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At the Institute for Medical Diagnostics, Nikolaistr. 22, D-12247 Berlin (inspected by DAKKS, Deutsche Akkreditierungsstelle GmbH, and accredited to DIN EN ISO/IEC 17025:2005 and DIN EN ISO 15189:2007), the FDOJ samples were homogenized by centrifugation in 200 μL of cold protease inhibitor buffer (Complete Mini Protease Inhibitor Cocktail; Roche Diagnostics GmbH, Penzberg, Germany). The homogenate was centrifuged for 15 min at 13,400 rpm. Following this, the supernatant was collected and centrifuged for a further 25 min at 13,400 rpm. In the 14 supernatants of tissue homogenate, levels of the following cytokines were measured: RANTES, FGF-2, interleukin (IL)-1 receptor antagonist (ra), IL-6, IL-8, monocyte chemotactic protein-1 (MCP1), and tumor necrosis factor-alpha (TNF-α). Measurement was performed using the Human Cytokine/Chemokine Panel I (MPXHCYTO-60K; Merck KGaA, Darmstadt, Germany) according to the manufacturer’s instructions and analyzed using the Luminex® 200™ with xPonent® Software (Luminex Co, Austin, TX, USA).
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