Plasma was collected from peripheral blood, spun at 3000 g for 2 min and stored at −20°C until assayed. IFN-γ levels throughout infection were quantified using the IFN-γ ‘Femto-HS’ High Sensitivity Mouse Uncoated ELISA Kit (Invitrogen), according to manufacturer instructions. The concentration of pro-inflammatory cytokines at day 6 PI was assessed by cytometric bead array (Legendplex custom multi-analyte flow assay kit, Biolegend) according to the manufacturer’s protocol.
Legendplex custom multi analyte flow assay kit
Manufactured by BioLegend
The LegendplexTM custom multi-analyte flow assay kit is a laboratory tool designed to facilitate the simultaneous measurement of multiple analytes in a single sample. The kit utilizes flow cytometry technology to enable the detection and quantification of various biomolecules, such as proteins, cytokines, or other targets of interest.
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Lab products found in correlation
2 protocols using legendplex custom multi analyte flow assay kit
1
Quantifying Cytokine Levels in Infection
2
Quantifying Cytokine Levels in Infection
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Plasma was collected from peripheral blood, spun at 3000 g for 2 min and stored at −20°C until assayed. IFN-γ levels throughout infection were quantified using the IFN-γ ‘Femto-HS’ High Sensitivity Mouse Uncoated ELISA Kit (Invitrogen), according to manufacturer instructions. The concentration of pro-inflammatory cytokines at day 6 PI was assessed by cytometric bead array (Legendplex custom multi-analyte flow assay kit, Biolegend) according to the manufacturer’s protocol.
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