HUVECs and SMCs (C-12205 and C-12511, PromoCell) were maintained in endothelial growth medium 2 (EGM2; C-22111, PromoCell) and SMC growth medium (SMCGM2; C-22062, PromoCell), respectively, under water-saturated 5% CO2 atmosphere at 37°C. Cells were grown in collagen-I–coated flasks (10190103, Thermo Fisher Scientific) and detached at passage 5 for vesseloid formation.
C 12511
Manufactured by PromoCell
Sourced in Germany
The C-12511 is a laboratory equipment designed for cell culture applications. It features temperature and humidity control to maintain optimal cell growth conditions. The device is suitable for a variety of cell types and can be used in research and clinical settings.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using c 12511
1
Vesseloid Formation from HUVECs and SMCs
2
Culture of Aortic SMCs and HEK293 Cells
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Human aortic SMCs (C-12511, Promocell, Karlsruhe, Germany) and HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (provided by Gibco Life Technologies, Gaithersburg, MD, USA), which contained 100 units/mL of penicillin, 100 μg/mL of streptomycin, and 10% FBS, and was low in glucose in a humidified incubator at 37 °C with 5% CO2.
3
Osteogenic Differentiation of Coronary Artery Smooth Muscle Cells
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Human coronary artery smooth muscle cells (hSMCs, C-12511, PromoCell) were cultured in SMC growth medium (C-22052, PromoCell) at 37°C with 5% CO2. Cells were inoculated on 24 or 48 well plates by 1 x 106 cells/ml with normal condition medium (NM, DMEM with 4.5 g/L glucose (10569010, Thermo Fisher Scientific containing 10% fetal bovine serum). After 24-h, medium was changed to osteogenic medium (OM, NM added with 10% fetal bovine serum, 10 mmol/L β-glycerophosphate disodium salt pentahydrate, 100 μmol/L L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate, 10 nmol/L dexamethasone). Each compound then was added in formulation of DMSO solution in 1-to-2,000-fold dilution. DMSO was used as control with NM and OM. Media containing compound was changed every 3-4 days.
4
Senescent and Damaged VSMC Cultures
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Human coronary smooth muscle cells from a young healthy donor were purchased from Promocell (C-12511) and cultured in growth medium (Promocell, C-22062), supplemented with 10% FBS and 1% penicillin-streptomicin (Sigma Aldrich). To achieve RS, cells were serially passaged (Bielak-Zmijewska et al., 2014 (link)). Cells were cultured until 70–80% confluence and re-seeded in T75 Flasks (Thermo Fisher Scientific, Nunc EasYFlask, #156499) at a density of 3.5 × 103 cells/cm2 until proliferative arrest (passage 6–8). Cumulative population doublings were counted using the formula cPD = X + 3.322∗(log Y−log I), X representing the cumulative population doubling of the subculture used to initiate the culture, Y being the viable cell number on harvest, and I the cell number on inoculation. Low passage VSMCs (passage 2–3) were treated with bleomycin to induce DS, as previously reported (Gardner et al., 2015 (link)).
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