BN-PAGE was performed as described previously (Bricker et al., 2012 (link)). Mitochondria were resuspended in lysis buffer (50 mM NaCl, 5 mM 6-aminocaproic acid, 50 mM imidazole, 1 mM AEBSF, and protease inhibitor cocktail). Mitochondria were solubilized with ~1.5% digitonin. Lysates were resolved on a 3%–13% gradient native gel using a PROTEAN®II xi Cell gel running system (Bio-Rad). Western blot performed as described elsewhere using a Trans-blot transfer cell (Bio-Rad) and PVDF membranes.
Protean 2 xi cell gel running system
Manufactured by Bio-Rad
The PROTEAN®II xi Cell gel running system is a laboratory equipment used for separating proteins or nucleic acids in an electrophoresis gel. It provides a controlled environment for the gel-based separation process.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using protean 2 xi cell gel running system
1
Native PAGE Analysis of Mitochondria
2
Native Gel Electrophoresis of Mitochondria
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