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Tcs sp5 2

Manufactured by Zeiss
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The TCS SP5 II is a confocal laser scanning microscope system designed for high-performance imaging applications. It features a modular design, allowing for customization to suit various research and analysis requirements. The system provides advanced optical capabilities, including multiple laser excitation sources, sensitive detectors, and sophisticated image acquisition and processing capabilities.

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Lab products found in correlation

Mitotracker red, by Thermo Fisher Scientific (3 mentions) Lsm 880, by Zeiss (2 mentions) Lsm 780, by Zeiss (2 mentions) Lsm780 inverted, by Zeiss (1 mentions) Eclipse te2000 u microscope, by Nikon (1 mentions) Nih imagej, by Adobe (1 mentions) Evos cell imaging system, by Thermo Fisher Scientific (1 mentions)

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TCS SP5 II confocal microscope TCS SP5 SP5 II

5 protocols using tcs sp5 2

1

Mitochondrial Redistribution Assay

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105 cells were grown on 18-mm cover slips coated with collagen. After various treatments, cells were fixed and stained with different antibodies and Hoechst 33258 dye, as described previously55 (link). For mitochondrial staining, cells were incubated with 100 nM MitoTracker Red (Thermo Fisher Scientific, MA, USA) for 15 min at 37 °C before fixation, as described9 (link). Fluorescent images were captured using a fluorescence microscope (EVOS Cell Imaging Systems, Thermo Fisher Scientific, MA, USA) or confocal microscope (LEICA TCS SP5 II) using Zeiss X63 NA 1.4 objective lens. To determine the number of cells exhibiting the redistribution effect, the fluorescently stained cells were counted under the fluorescence microscope.
Lindenboim L., Grozki D., Amsalem-Zafran A.R., Peña-Blanco A., Gundersen G.G., Borner C., Hodzic D., Garcia-Sáez A.J., Worman H.J, & Stein R. (2020). Apoptotic stress induces Bax-dependent, caspase-independent redistribution of LINC complex nesprins. Cell Death Discovery, 6, 90.
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2

Quantifying Fluorescence Intensity in Tissue Samples

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Photomicrographs were taken with Nikon Eclipse-TE-2000U microscope at 20× magnification. Alternatively, for confocal imaging, a Leica TCS SP5 II or a Zeiss LSM-780 inverted microscope (z-stacks, slice spacing 1.65μm) were used at 20× or 63x magnification. DRG, spinal cord and sciatic nerve micrographs were processed with the software ImageJ: a constant fluorescence intensity threshold was set across each cell (or area) in control-tissues. On the basis of this threshold, for each cell (or area) in the samples, the intensity of pixels was calculated in each channel. This was done in triplicate and the investigator was blinded to the experimental group.
La Montanara P., Hervera A., Baltussen L., Hutson T.H., Palmisano I., De Virgiliis F., Kong G., Chadwick J., Gao Y., Bartus K., Majid Q.A., Gorgoraptis N., Wong K., Downs J., Pizzorusso T., Ultanir S., Leonard H., Yu H., Millar D.S., Istvan N., Mazarakis N.D, & Giovanni S.D. (2020). Cyclin-dependent-like kinase 5 is required for pain signaling in human sensory neurons and mouse models. Science translational medicine, 12(551), eaax4846.
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3

Fluorescent Imaging of Labeled Cells

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Fluorescent images of immunostained cells or transgenic animals expressing GFP and/or mCherry reporters were captured using a Leica TCS SP5 II or Zeiss LSM 880 laser confocal microscope.
Hu Y., Xu Z., Pan Q, & Ma L. (2023). Casein kinase 1 gamma regulates oxidative stress response via interacting with the NADPH dual oxidase complex. PLOS Genetics, 19(4), e1010740.
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4

Confocal Imaging of CNS and Legs

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Multiple 1-μm-thick sections in the z axis (dorsoventral for L3 CNS or adult VNC and mediolateral for adult legs) were imaged with a Leica TCS SP5 II or a Zeiss LSM 780 confocal microscope. Binary images for z stack images were generated using NIH ImageJ and Photoshop (Adobe Systems).
Enriquez J., Rio L.Q., Blazeski R., Bellemin S., Godement P., Mason C, & Mann R.S. (2018). Differing Strategies Despite Shared Lineages of Motor Neurons and Glia to Achieve Robust Development of an Adult Neuropil in Drosophila. Neuron, 97(3), 538-554.e5.
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5

Quantifying Apoptotic Events in Cultured Cells

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105 cells were seeded and grown on 18-mm cover slips coated with collagen. After treatments, cells were fixed and stained with the indicated antibodies together with Hoechst 33258 dye, as described previously [48 (link)]. Cells were co-stained with anti-nesprin-2G and anti-Bax 6A7 or with anti-cytochrome c and anti-Bak NT Abs. Images were captured using a fluorescence microscope (EVOS Cell Imaging Systems, Thermo Fisher Scientific) or confocal microscope (LEICA TCS SP5 II) using Zeiss X63 NA 1.4 objective lens.
Cells exhibiting the indicated apoptotic events were counted under the fluorescence microscope. In the case of Bak-NT staining in healthy Bcl-xL−/− MEFs or WT MEFs treated with Bcl-xL and nesprin-2 siRNAs, 15 random images from each treatment group were captured using confocal microscope, and the number of cells in the different treatments exhibiting Bak-NT staining pattern of elongated structures were blindly counted.
Lindenboim L., Zohar H., Gundersen G.G., Worman H.J, & Stein R. (2024). LINC complex protein nesprin-2 has pro-apoptotic activity via Bcl-2 family proteins. Cell Death Discovery, 10, 29.
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