Accu chek active blood glucose test strips

The blood glucose and plasma insulin levels were measured using Accu-Chek Active Blood Glucose Test Strips (Roche Diagnostics, Indianapolis, IN, USA) and enzyme-linked immunosorbent assay (ELISA) kits (TMB Mouse Insulin ELISA kit, Shibayagi, Gunma, Japan), respectively. The level of hepatic glycogen was determined using the anthrone-H₂SO₄ method with glucose as standard (29 (link)). The homeostasis model assessment of insulin resistance (HOMA-IR) index was calculated using the equation described by Vogeser et al. (30 (link)). The following plasma adipokines were analyzed using commercial assay kits: adiponectin (Shibayagi), leptin (Cayman Chemical, Ann Arbor, MI, USA), resistin (B-Bridge International, Osaka, Japan), and tumor necrosis factor (TNF)-α (Abcam, Cambridge MA, USA).

ACCU-CHEK Active blood glucose test strips were obtained from Roche (NJ, USA). Triglyceride (TG) and total cholesterol (TC) levels, as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and malondialdehyde (MDA) activities, were measured using kits from Jiancheng Bioengineering Institute (Nanjing, China). Enzyme-linked immunosorbent assay (ELISA) kits specific for collagen I, cardiac troponin I (cTn-I), tumor necrosis factor-α (TNF-α), IL-1β, and IL-6 were obtained from HaiTai TongDa Sci Tech Ltd (Beijing, China). Antibodies specific for caspase-3, caspase-9, Bcl-2, Bax, NFκB, AKT, p-AKT, β-actin, and lamin B were obtained from Abcam (MA, USA). horseradish peroxidase (HRP)-conjugated secondary antibodies were obtained from CW Biotech (Beijing, China). All other chemicals were obtained from Sigma (St. Louis, MO, USA) and were of analytical grade or higher.