Rabbit anti sr b1

iHLCs were fixed with −20°C methanol for 10 min at 4°C, washed thrice with PBS, blocked with 2% BSA in PBS overnight at 4°C, and then incubated overnight at 4°C with a primary antibody: mouse anti-human CD81 (clone JS-81, BD Pharmingen; 1:200), rabbit anti-SRB1 (Novus Biologicals; 1:100), mouse anti-PbHSP70 (clone 2E6; 1:200 for P. berghei and P. yoelii), rabbit anti-PbMSP1 (1:500 for P. berghei), mouse anti-PfHSP70 (clone 4C9, Sanaria; 1:200 for P. falciparum), or mouse anti-PfMSP1 (1:200 for P. falciparum). Samples were washed thrice with PBS before incubation for 1–3 hr at room temperature with secondary antibody: goat anti-mouse Alexa Fluor 594 or Alexa Fluor 488 or donkey anti-rabbit-Alexa Fluor 488 (Invitrogen; 1:400). Nuclei were then counterstained with Hoechst 33258 (Invitrogen; 1:1000), samples were washed thrice with PBS, and 1 ml of Aquamount (Thermo-Scientific) was added per well. Images were captured on a Nikon Eclipse Ti fluorescence microscope or an Olympus FV1000 multiphoton laser scanning confocal microscope.