Ab98952

Manufactured by BD

Ab98952 is a laboratory equipment designed for general use in research and analytical settings. It is a versatile instrument that can perform a range of tasks required in a laboratory environment. The core function of this product is to provide reliable and accurate measurements or processing of samples, as per the standard protocols and procedures followed in the field of study.

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Lab products found in correlation

Lsm 700 confocal microscope, by Zeiss (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Sc 13024, by Abcam (1 mentions) C8035, by Abcam (1 mentions) Smi 312r, by Santa Cruz Biotechnology (1 mentions) Mab1997, by Merck Group (1 mentions) Mab3420, by Merck Group (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Ab5054, by Merck Group (1 mentions) Cytaion5, by Agilent Technologies (1 mentions) Af748, by R&D Systems (1 mentions) Hoechst 33258, by Thermo Fisher Scientific (1 mentions) Leica tcs sp5, by Leica camera (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)

2 protocols using ab98952

Immunofluorescence Markers for Neural Characterization

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For immunofluorescence, we used rabbit anti-C3G (Santa Cruz Biotechnology, H-300/sc-15359, 1:150), mouse anti-N-Cadherin (Abcam # ab98952, 1:200), mouse anti-Nestin (BD Biosciences #611658, 1:200), rabbit anti-NF medium chain (Abcam #ab64300, 1:200) or mouse anti-NF medium chain (2H3, DSHB, 1:4), rabbit anti-NF light chain (Cell Signaling #2837, 1:200), mouse anti-SMI 312 (Covance SMI-312R, 1:200), rabbit anti-Cux1 (Santa Cruz Biotechnology #sc13024, 1:150), rabbit anti-Tbr1 (Abcam #31940, 1:400), rabbit anti-Tbr2 (Abcam #23345, 1:400), rat anti-β1 integrin [very late antigen (VLA), Chemicon, #MAB1997, 1:200], rabbit anti-Calretinin (Millipore #AB5054, 1:1000), mouse anti-chondroitin sulfate (clone CS-56, Sigma #C8035, 1:100), rabbit anti-Tbr1 (Abcam #ab31940, 1:500), mouse Tau-1 (Chemicon #MAB3420; 1:500), mouse anti-MAP2 (Chemicon #AB5622; 1:1000), Hoechst 33342 (Molecular probes, 1:6000) and goat secondary antibodies labeled with Alexa 488 or 594 (Molecular Probes, 1:800). The rat 9EG7 (supernatant) antibody was provided by D. Vestweber (Max Planck Institute for Molecular Biomedicine, Münster, Germany). Images were taken on a Zeiss LSM 700 confocal microscope using the Zeiss ZEN software.

Shah B., Lutter D., Bochenek M.L., Kato K., Tsytsyura Y., Glyvuk N., Sakakibara A., Klingauf J., Adams R.H, & Püschel A.W. (2016). C3G/Rapgef1 Is Required in Multipolar Neurons for the Transition to a Bipolar Morphology during Cortical Development. PLoS ONE, 11(4), e0154174.

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Immunofluorescence Staining of Tissues and Cells

Cited 2 times

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Tissues and tumors were fixed in 10% formalin and cells from culture were fixed in 4% paraformaldehyde and stained as described [6 , 14 (link), 15 (link)]. Primary antibodies were as follows: anti-E-cadherin R&D AF748 (1:100); anti-N-Cadherin Abcam ab98952 (1:100); anti-CD3 BD Pharmingen 550275 (1:100). The secondary antibodies were as follows: Donkey Alexa 488-conjugated IgG or Cy3-conjugated IgG. Nuclei were stained with DAPI or Hoechst 33258 (Invitrogen). Images were acquired with a confocal Leica TCS SP5 (Leica), Carl Zeiss upright Apotome Axio Imager Z1 or Cytaion5 (Biotek).

Su F., Ahn S., Saha A., DiGiovanni J, & Kolonin M.G. (2018). Adipose stromal cell targeting suppresses prostate cancer epithelial-mesenchymal transition and chemoresistance. Oncogene, 38(11), 1979-1988.

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