The MEK inhibitor PD184352, the AKT inhibitor AKTi, Epidermal Growth Factor (EGF), basic Fibroblast Growth Factor (bFGF), Polyethylenimine (PEI), Avidin-FITC and 4′6-diamino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich (Rehovot, Israel). Protein A/G PLUS-agarose beads were obtained from Santa Cruz Biotechnology, Inc. (CA, USA). Glutathione beads were purchased from GE-healthcare and Proximity ligation assay kit was from Olink Bioscience (both Uppsala, Sweden). Albumin bovine serum (BSA) was purchased from MP biomedical (OH, USA). Si RNAs and Dharmafect were from Thermo Fisher Scientific (CO, USA). NBT/BCIP developing substrates were purchased from Promega (Wis, USA). Soybean trypsin inhibitor (SBTI) was purchased from Biological Industries (Beit HaEmek, Israel).
Proximity ligation assay kit
Manufactured by Olink
Sourced in Sweden
The Proximity Ligation Assay (PLA) kit is a laboratory tool used to detect and quantify protein-protein interactions. It allows researchers to investigate the physical proximity of two target proteins within a sample, providing insights into cellular processes and signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Nbt bcip developing substrates, by Promega (2 mentions)
Dharmafect, by Thermo Fisher Scientific (2 mentions)
4 6 diamino 2 phenylindole dapi, by Merck Group (2 mentions)
Sirnas, by Thermo Fisher Scientific (2 mentions)
Polyethylenimine (pei), by Merck Group (2 mentions)
Avidin fitc, by Merck Group (1 mentions)
Glutathione beads, by GE Healthcare (1 mentions)
Albumin bovine serum bsa, by MP Biomedicals (1 mentions)
Protein a g plus agarose bead, by Santa Cruz Biotechnology (1 mentions)
Pd184352, by Merck Group (1 mentions)
Epidermal growth factor (egf), by Merck Group (1 mentions)
Tetradecanoyl phorbol acetate, by Merck Group (1 mentions)
Calf intestinal phosphatase, by New England Biolabs (1 mentions)
2 protocols using proximity ligation assay kit
1
Investigating Cellular Signaling Pathways
2
Molecular Interaction Detection Toolkit
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tetradecanoyl phorbol acetate (TPA), anisomicin, Polyethylenimine (PEI) and 4'6-diamino-2phenylindole (DAPI) were obtained from Sigma (Rehovot, Israel). A/G beads were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Albumin bovine serum (BSA) was purchased from MP niomedical (Solon, OH, USA). Si RNAs and Dharmafect were from Thermo Fisher Scientific (CO, USA). Proximity ligation assay kit was from Olink Bioscience (Uppsala, Sweden). NBT/BCIP developing substrates were purchased from Promega (WI, USA), Calf Intestinal Phosphatase (CIP) was from New England Biolabs (MA, USA).
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