Cellgro penicillin streptomycin solution

All lipids were purchased from Avanti Polar lipids (Alabaster, AL, USA). Dialysis membranes were purchased from Spectrum Labs (Rancho Dominquez, CA, USA). PD-10 pre-packed Sephadex^™G-25M gel filtration columns were purchased from GE Healthcare (Knox, IN, USA). Bis-dPEG®₂-NHS ester and Bis-dPEG®₄-NHS ester were purchased from Quanta Biodesign (Plain City, OH, USA). All other reagents and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used without further purification. Chinese Hamster Ovary (CHO-K1) cells were purchased from American Type Culture Collection (Manassas, VA, USA). RPMI and F-12K media were also purchased from American Type Culture Collection (Manassas, VA, USA). Fetal bovine serum was purchased from Atlanta Biologicals Inc., (Flowery Branch, GA, USA). Corning cellgro® Penicillin Streptomycin solution was purchased from Corning Inc. (Corning, NY, USA).

Chinese Hamster Ovary (CHO-K1) cells were purchased from American Type Culture Collection (Manassas, VA, USA). F-12K media was also purchased from American Type Culture Collection. Fetal bovine serum was purchased from Atlanta Biologicals Inc. (Flowery Branch, GA, USA). Corning Cellgro® Penicillin–Streptomycin solution was purchased from Corning Inc. (Corning, NY, USA). Lipofectamine® 2000 Transfection Reagent and OptiMEM® media were purchased from Life Technologies (Grand Island, NY, USA). Endotoxin-Free EGFP plasmid DNA (GFP-P) was purchased from Addgene (Cambridge, MA, USA). The ZnBDPA compounds 1 and 2 were synthesized as described previously.³⁶ All other reagents and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used without further purification.