DMEM/F12 high glucose was provided by Hyclone (Utah, USA). Fatal bovine serum(FBS), trypsin, penicillin-streptomycin, Annix-V/PI kit, Cell Counting Kit-8(CCK-8), JC-1 kit and ROS kit were obtained from Gibco (New York, USA). Transwell chambers with membranes at a pore size of 8 μm were bought from Corning Life Science (New York, USA). Hoechst 33258 was purchased from Sigma-Aldrich (St Louis, MO, USA). PI was purchased from MultiSciences (Zhejiang Province, China). Primary antibodies against p53, p27, cleaved caspase-9, cleaved caspase-3, Bcl-2, Bax, cyclinD1, cyclin-dependent kinase 2 (CDK2), cytochrome c, apoptotic protease activating factor 1 (Apaf-1), LC3-I, LC3-II and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were provided by Cell Signaling Technology (Beverly, MA, USA). Horseradish peroxidase (HRP)-conjugated secondary antibodies were provided by Huabio (Hangzhou, Zhejiang, China). Pifithrin-α (PFT-α) was provided by Selleck Chemicals (Shanghai, China). PNS was provided by Kunming Pharmaceutical Company (Kunming, China).
Annix 5 pi kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Annix-V/PI kit is a reagent used to detect and quantify apoptosis (programmed cell death) in cells. The kit utilizes Annexin V, a calcium-dependent phospholipid-binding protein, and propidium iodide (PI), a DNA-binding dye, to distinguish between viable, early apoptotic, and late apoptotic/necrotic cells.
Automatically generated - may contain errors
Lab products found in correlation
Cleaved caspase 3, by Cell Signaling Technology (2 mentions)
Cell counting kit 8 cck 8, by Thermo Fisher Scientific (2 mentions)
Hoechst 33258, by Merck Group (2 mentions)
Jc 1 kit, by Thermo Fisher Scientific (2 mentions)
Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Cell Signaling Technology (2 mentions)
Bcl 2, by Cell Signaling Technology (2 mentions)
Cleaved caspase 9, by Cell Signaling Technology (2 mentions)
Cytochrome c, by Cell Signaling Technology (2 mentions)
Lc3 2, by Cell Signaling Technology (2 mentions)
Ros kit, by Thermo Fisher Scientific (2 mentions)
Apoptotic protease activating factor 1 apaf 1, by Cell Signaling Technology (2 mentions)
Horseradish peroxidase hrp conjugated secondary antibodies, by Huabio (2 mentions)
Lc3 1, by Cell Signaling Technology (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fatal bovine serum, by Thermo Fisher Scientific (1 mentions)
Cyclin d1, by Cell Signaling Technology (1 mentions)
Cyclin dependent kinase 2, by Cell Signaling Technology (1 mentions)
Pifithrin α pft α, by Selleck Chemicals (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Transwell chambers with 8 μm pore sized membranes, by Corning (1 mentions)
2 protocols using annix 5 pi kit
1
Assessing Apoptosis and Autophagy in Cells
2
Apoptosis Pathway Regulation in Cells
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DMEM/F12 high glucose was provided by Hyclone (Utah, USA), fatal bovine serum(FBS), trypsin ,penicillin-streptomycin, Annix-V/PI kit, Cell Counting Kit-8(CCK-8), JC-1 kit and ROS kit were supplied by Gibco (New York, USA), transwell chambers with 8 μm-pore-sized membranes were bought from Corning Life Science (New York, USA), Hoechst 33258 was purchased from Sigma-Aldrich (St Louis, MO, USA), PI was purchased from MultiSciences (Zhejiang Province, China), primary antibodies against p53, p27,cleaved caspase-9, cleaved caspase-3, Bcl-2, Bax, cyclinD1, cyclin-dependent kinase 2 (CDK2), cytochrome c, apoptotic protease activating factor 1 (Apaf-1), LC3-I, LC3-II, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were provided by Cell Signaling Technology (Beverly, MA, USA), horseradish peroxidase (HRP)-conjugated secondary antibodies were provided by Huabio (Hangzhou, Zhejiang, China), pi thrin-α (PFT-α) was provided by Selleck Chemicals (Shanghai, China), PNS was provided by Kunming Pharmaceutical Company (Kunming, China).
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