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1.4 planapochromat objectives

Manufactured by Olympus
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The 1.4 PlanApochromat objectives from Olympus are high-performance microscope objectives designed to provide excellent optical performance. These objectives are engineered to deliver a flat, distortion-free image with accurate color reproduction across a wide field of view.

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Lab products found in correlation

Softworx acquisition and deconvolution software, by GE Healthcare (2 mentions) Optically clear polymer bottom μ dishes, by Ibidi (2 mentions) Phenol red free co2 independent medium, by Thermo Fisher Scientific (2 mentions) Hoechst no 33342, by Merck Group (2 mentions) Deltavision core widefield fluorescence system, by Olympus (2 mentions) Coolsnap charge coupled device camera, by Teledyne (2 mentions)

2 protocols using 1.4 planapochromat objectives

1

Live Cell Microscopy Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
For live imaging, cells were cultured in 35 μm optically clear polymer-bottom μ-dishes (ibidi) and transiently transfected for 18 hrs using PEI. Growth medium was replaced with Phenol Red-free CO2 independent medium (ThermoFisher) and DNA stained by incubating the cells for 20 min at 37°C in medium containing 0.25 μg/m Hoechst No. 33342 (Sigma-Aldrich). Images were acquired using a DeltaVision CORE widefield fluorescence system fitted with 40x NA 1.2 and 60× NA 1.4 PlanApochromat objectives (Olympus), CoolSNAP charge-coupled device (CCD) camera (Roper Scientific) and environmental chamber. The microscope was controlled and images processed by SoftWorX acquisition and deconvolution software (GE Healthcare). All images are single, deconvolved optical sections.
Srivastava G., Bajaj R., Kumar G.S., Gaudreau-Lapierre A., Nicolas H., Chamousset D., Kreitler D., Peti W., Trinkle-Mulcahy L, & Page R. (2022). The ribosomal RNA processing 1B:protein phosphatase 1 holoenzyme reveals non-canonical PP1 interaction motifs. Cell reports, 41(9), 111726.
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2

Live Cell Microscopy Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
For live imaging, cells were cultured in 35 μm optically clear polymer-bottom μ-dishes (ibidi) and transiently transfected for 18 hrs using PEI. Growth medium was replaced with Phenol Red-free CO2 independent medium (ThermoFisher) and DNA stained by incubating the cells for 20 min at 37°C in medium containing 0.25 μg/m Hoechst No. 33342 (Sigma-Aldrich). Images were acquired using a DeltaVision CORE widefield fluorescence system fitted with 40x NA 1.2 and 60× NA 1.4 PlanApochromat objectives (Olympus), CoolSNAP charge-coupled device (CCD) camera (Roper Scientific) and environmental chamber. The microscope was controlled and images processed by SoftWorX acquisition and deconvolution software (GE Healthcare). All images are single, deconvolved optical sections.
Srivastava G., Bajaj R., Kumar G.S., Gaudreau-Lapierre A., Nicolas H., Chamousset D., Kreitler D., Peti W., Trinkle-Mulcahy L, & Page R. (2022). The ribosomal RNA processing 1B:protein phosphatase 1 holoenzyme reveals non-canonical PP1 interaction motifs. Cell reports, 41(9), 111726.
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