Cells were fixed in 4% paraformaldehyde (EM grade, Electron Microscopy Sciences) for 10 min, rinsed three times in PBS and permeabilized for 10 min in PBS/0.2% Triton X-100 at room temperature, followed by three rinses in PBS. After blocking for 30 min at room temperature in PBS/5% FBS, slides were incubated with the primary antibody against DDX20 (mouse monoclonal, sc-57007, Santa Cruz Biotechnology, 1:200 dilution) or SMN (rabbit polyclonal, sc-15320, Santa Cruz Biotechnology, 1:200 dilution) in PBS/5% FBS at 4°C overnight. After three rinses in PBS, slides were incubated with the secondary antibody (1:1,000 dilution in both cases) and Hoechst nuclear dye (1:10,000 dilution) for 90 min at room temperature. The secondary antibody for DDX20 was a goat anti-mouse Alexa 594 antibody (Invitrogen) and the secondary antibody for SMN was a donkey anti-rabbit Alexa 568 antibody (Invitrogen). Slides were rinsed three times in PBS and once in water. If no FISH was performed subsequently, slides were mounted.
Sc 57007
Manufactured by Santa Cruz Biotechnology
Sc-57007 is a laboratory equipment product offered by Santa Cruz Biotechnology. It serves as a general-purpose tool for researchers and scientists in various fields. The core function of Sc-57007 is to assist in the collection, processing, and analysis of samples or data. No further details on the intended use or specific applications of this product are provided.
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Lab products found in correlation
2 protocols using sc 57007
1
Immunofluorescence Staining for DDX20 and SMN
2
Immunofluorescence Staining for DDX20 and SMN
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Cells were fixed in 4% paraformaldehyde (EM grade, Electron Microscopy Sciences) for 10 min, rinsed three times in PBS and permeabilized for 10 min in PBS/0.2% Triton X-100 at room temperature, followed by three rinses in PBS. After blocking for 30 min at room temperature in PBS/5% FBS, slides were incubated with the primary antibody against DDX20 (mouse monoclonal, sc-57007, Santa Cruz Biotechnology, 1:200 dilution) or SMN (rabbit polyclonal, sc-15320, Santa Cruz Biotechnology, 1:200 dilution) in PBS/5% FBS at 4°C overnight. After three rinses in PBS, slides were incubated with the secondary antibody (1:1,000 dilution in both cases) and Hoechst nuclear dye (1:10,000 dilution) for 90 min at room temperature. The secondary antibody for DDX20 was a goat anti-mouse Alexa 594 antibody (Invitrogen) and the secondary antibody for SMN was a donkey anti-rabbit Alexa 568 antibody (Invitrogen). Slides were rinsed three times in PBS and once in water. If no FISH was performed subsequently, slides were mounted.
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