Cfi plan fluor c elwd dic m

A WITec Alpha 300 RSA+ confocal microscope was used to record Raman spectra and imaging. The configuration of experimental set up was as follows: the diameter of fiber: 50 μm for 355 and 532 nm and 100 μm for 785 nm, an Acton-SP-2300i monochromator (Acton, MA, USA) and a DU970-UVB-353 CCD camera for 355 and 532 nm (Andor Newton, Belfast, Northern Ireland) and an Ultra High Throughput Spectrometer (UHTS 300, WITec, Ulm, Germany) and an Andor Newton iDU401A-BR-DD-352 CCD camera for 785 nm. Excitation lines were focused on the sample through a 40x dry objective (Nikon, Warszawa, Poland) objective type CFI Plan Fluor C ELWD DIC-M, numerical aperture (NA) of 0.60 and a 3.6–2.8 mm working distance). The average laser excitation power was 1 mW for 355 nm, 10 mW for 532, 10 mW for 633 nm, and 80 mW for 785 nm, with an integration time of 0.5 s, 0.5 s/1.0 s, 2.0 s and 0.5 s respectively. An edge filters were used to remove the Rayleigh scattered light. A piezoelectric table was used to record Raman images. No pre-treatment of the samples was necessary before Raman measurements. The cosmic rays were removed from each Raman spectrum (model: filter size: 2, dynamic factor: 10) and the smoothing procedure: Savitzky–Golay method was also implemented (model: order: 4, derivative: 0). Data acquisition and processing were performed using the WITec Project Plus software.

A WITec (Ulm, Germany) alpha 300 RSA+ confocal microscope was used to record Raman spectra and imaging. The configuration of the experimental set-up was as follows: the diameter of fiber: 50 μm, a monochromator Acton-SP-2300i and a CCD camera Andor Newton DU970-UVB-353, the excitation laser line 532 nm. The excitation line was focused on the sample through a 40× dry objective (Nikon, objective type CFI Plan Fluor C ELWD DIC-M, numerical aperture (NA) of 0.60 and a 3.6–2.8 mm working distance). The average laser excitation power was 10 mW, with an integration time of 0.5 s. Edge filters were used to remove the Rayleigh scattered light. A piezoelectric table was used to record Raman images. No pre-treatment of the samples was necessary before Raman measurements. The cosmic rays were removed from each Raman spectrum (model: filter size: 2, dynamic factor: 10) and the smoothing procedure, Savitzky–Golay method, was also implemented (model: order: 4, derivative: 0). Data acquisition and processing were performed using WITec Project Plus software.