Thiacalix[4]arene tetrasulfonate (TCAS) with purity greater than 98% was purchased from TCI America (city, state abbrev if USA, country). α-Naphthoflavone and β-NADPH were purchased from Roche Molecular Biochemicals (Basel, Switzerland). Recombinant human cytochromes P450 (CYP1A2, 3A5, 2C9, 2D6, 2E1 and 3A4), pooled human liver microsomes (HLMs) and CYP450 monoclonal antibodies (CYP1A2, 2C9, 2D6 and 3A4) were provided by BD Gentest (Woburn, MA, USA). All other reagents and solvents were of analytical grade and commercially available.
Pooled human liver microsomes hlms
Manufactured by BD
Sourced in United States
Pooled human liver microsomes (HLMs) are a preparation of subcellular fractions derived from pooled human liver tissue. They contain the microsomal fraction, which is enriched in drug-metabolizing enzymes, primarily cytochrome P450 enzymes. HLMs can be used to study in vitro drug metabolism and drug-drug interactions.
Automatically generated - may contain errors
Lab products found in correlation
β nadph, by Roche (1 mentions)
Glucose 6 phosphate (g6p), by Merck Group (1 mentions)
Glucose 6 phosphate dehydrogenase, by Merck Group (1 mentions)
Mephenytoin, by Merck Group (1 mentions)
Phenacetin, by Merck Group (1 mentions)
Chlorzoxazone, by Merck Group (1 mentions)
Dextromethorphan, by Merck Group (1 mentions)
Paclitaxel, by Merck Group (1 mentions)
Testosterone, by Merck Group (1 mentions)
Diclofenac, by Merck Group (1 mentions)
Coumarin, by Merck Group (1 mentions)
Prednisolone, by Meilun (1 mentions)
Ascomycin, by Meilun (1 mentions)
4 protocols using pooled human liver microsomes hlms
1
Cytochrome P450 Inhibition Assay
2
Isolation and Synthesis of Polyozellin Compounds
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Polyozellin was isolated from Polyozellus multiplex (Black chanterelle), and thelephoric acid was prepared from polyozellin (Fig. 1 ). Pooled human liver microsomes (HLMs) were obtained from BD Gentest (Woburn, MA, USA). Glucose-6-phosphate, B-NADPH, and Glucose-6-phosphate dehydrogenase were purchased from Sigma (St. Louis, MO, USA). All other chemicals were of analytical grade and used as received.
3
Pogostone and Hepatic Metabolism
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Pogostone was obtained from the National Institutes for Food and Drug Control (Beijing, China). The substrates, including phenacetin, testosterone, coumarin, chlorzoxazone, dextromethorphan, diclofenac, mephenytoin, and paclitaxel, were obtained from Sigma Chemical Co. (Chicago, IL, USA). Pooled human liver microsomes (HLMs) were purchased from BD Biosciences (Woburn, MA, USA). The purity of chemicals was more than 98%, and other reagents were of analytical reagent grade.
4
Schizandrol A and B CYP3A Metabolism
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Schizandrol A (purity ≥ 98%, lot: 58546–56-8) and Schizandrol B (purity ≥ 98%, lot: 58546–55-7) were purchased from PUSH Bio-Technology Co., Ltd. (Chengdu, China). CYP3cide (PF-4981517, lot: ECD192–1-PFZ), 6β-hydroxytestosterone (purity ≥ 98%, lot: A0277795) and nicotinamide adenine dinucleotide phosphate (NADPH, lot: LA50Q51) were obtained from J&K Technology Co., Ltd. (Beijing, China). Tacrolimus (purity ≥ 99.0%, lot: 050701130614) and testosterone (purity ≥ 99.0%, lot: L1415024) were obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Ascomycin (purity ≥ 99.0%, lot: D1202AS) and prednisolone (purity ≥ 98%, lot: J0402AS) were from Dalian Meilun Bio-Technology Co., Ltd. (Dalian, China). Pooled human-liver microsomes (HLMs, lot: 4133007) from 22 donors were obtained from BD Biosciences Co., Ltd. (New York, NY, USA). CYP3A5 *1/*3 HLMs (lot: 0710232) and CYP3A5 *3/*3 HLMs (lot: 0710253) were purchased from Transheep Co., Ltd. (Shanghai, China). All other reagents and solvents were from standard chemical suppliers and of analytical grade.
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