Human ovarian cancer cells ES-2 and NIH : OVCAR-3 were obtained from American Type Culture Collection (ATCC). HO-8910 and HO-8910PM cells were kindly provided by Cell Bank/Stem Cell Bank, Chinese Academy of Sciences (Shanghai, China). SK-OV-3 cells was obtained from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China).
Skov 3
Manufactured by Shanghai Zhong Qiao Xin Zhou Biotechnology
Sourced in China
The SKOV-3 is a cell line derived from a human ovarian adenocarcinoma. It is commonly used in research applications to study ovarian cancer.
Automatically generated - may contain errors
Lab products found in correlation
Skov3, by Thermo Fisher Scientific (2 mentions)
Nih ovcar3, by American Type Culture Collection (1 mentions)
A2780, by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions)
Mccoy s 5a medium, by Thermo Fisher Scientific (1 mentions)
Hek293t, by Keygen Biotech (1 mentions)
Dulbecco modified eagle medium (dmem), by Keygen Biotech (1 mentions)
A2780, by Keygen Biotech (1 mentions)
Ovcar3, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Caov3, by Thermo Fisher Scientific (1 mentions)
Ovcar3, by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions)
Hyclone, by Cytiva (1 mentions)
Rpmi 1640 medium, by Cytiva (1 mentions)
A2780, by Cytiva (1 mentions)
Bovine insulin, by Solarbio (1 mentions)
Caov 3, by Procell (1 mentions)
Ovcar 3, by Cytiva (1 mentions)
Mccoy s 5a medium, by Procell (1 mentions)
Ovcar3, by Procell (1 mentions)
A2780, by Procell (1 mentions)
4 protocols using skov 3
1
Cell Line Acquisition for Ovarian Cancer
2
Cell Culture of Ovarian Cancer Lines
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The human ovarian cancer cell lines SKOV3 and A2780 were purchased from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. The HEK293 human embryonic kidney cell line (HEK293T) was a kind gift from Professor Chenbo Ji (Nanjing Medical University, Nanjing, China). The SKOV3 cells were cultured in McCoy's 5a medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS). The A2780 and HEK293T cells were cultured in Dulbecco's modified Eagle's medium (DMEM, KeyGEN BioTECH, Nanjing, China) supplemented with 10% FBS. All cell lines were maintained in a humidified atmosphere containing 5% CO2 at 37°C.
3
Cultivation of Ovarian Cancer Cell Lines
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Human ovarian clear cell cancer cell line ES-2 (ZQ0067) and ovarian adenocarcinoma cell line CAOV-3 (ZQ0484) were acquired from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd, and adenocarcinoma cell types SKOV-3 (TCHu185) and OVCAR-3 (TCHu228) were obtained from the National Collection of Authenticated Cell Cultures, Chinese Academy of Sciences. All cells were cultured in complete medium (McCoy's 5A for ES-2 and SKOV-3, DMEM for CAOV-3, and RPMI-1640 for OVCAR-3) containing 10% fetal bovine serum (FBS, Gibco) and 1% penicillin‐streptomycin, and maintained in 37 ℃ and 5% CO2 incubator.
4
Ovarian Cancer Cell Line Cultivation
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The ovarian cancer cell lines A2780, ES-2, OVCAR-3 and Caov-3 were purchased from Procell Life Science & Technology Co., Ltd. SKOV-3 and 293T cells were obtained from Shanghai Zhongqiaoxinzhou Biotechnology Co., Ltd. A2780, Caov-3 and 293T cells were cultured in DMEM (HyClone; Cytiva) supplemented with 10% FBS (Biological Industries). ES-2 cells were cultured in McCoy's 5A medium (Procell Life Science & Technology Co., Ltd.) supplemented with 10% FBS. OVCAR-3 cells were cultured in RPMI-1640 medium (HyClone; Cytiva) supplemented with 20% FBS and 0.01 mg/ml bovine insulin (Beijing Solarbio Science & Technology Co., Ltd.). SKOV-3 cells were cultured in RPMI-1640 medium supplemented with 15% FBS. All cells were incubated at 37˚C in an incubator with 5% CO2.
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