G1316b column compartment

Manufactured by Agilent Technologies

Sourced in Germany

The G1316B column compartment is a component of Agilent's liquid chromatography systems. Its core function is to precisely control and maintain the temperature of chromatographic columns during analysis.

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Lab products found in correlation

G1367d autosampler, by Agilent Technologies (1 mentions) Securityguard ultra guard column, by Phenomenex (1 mentions) Hplc grade water, by Thermo Fisher Scientific (1 mentions) Kinetex evo c18 column, by Phenomenex (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) 1200 series hplc system, by Agilent Technologies (1 mentions) G1315c dad, by Agilent Technologies (1 mentions) Chemstation lc 3d rev b 04, by Agilent Technologies (1 mentions) G1315c diode array detector, by Agilent Technologies (1 mentions) 1200 hplc system, by Agilent Technologies (1 mentions)

2 protocols using g1316b column compartment

HPLC Analysis of Phytocannabinoids

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Agilent Technologies 1200 Series HPLC system equipped with a G1315C DAD, G1316B column compartment, G1367D autosampler, and G1312B binary pump was used to analyse the acidic and neutral forms of phytocannabinoids. The separation was performed on a Phenomenex Kinetex EVO C18 column (5 μm, 100 x 2.1 mm id) with a Phenomenex SecurityGuard ULTRA guard column. Instrument control, data acquisition, and integration were done with ChemStation LC 3D Rev B.04.02 software (Agilent Technologies). A 2 μL injection volume was used for all calibration standards (THC, CBD, THC-A, CBD-A, CBG, CBG-A, all Sigma-Aldrich) and sample analysis. The compound peaks were detected for 230 nm and 280 nm. Mobile phases consisted of 50 mM ammonium formate (pH 5.19) (Sigma-Aldrich) in HPLC grade water (Fisher Chemical) on the A side and 100% methanol (Fisher Chemical) on the B side, with a flow rate 0.3 ml/min. Two samples per cultivar were analyzed, with two technical repeats per each sample. Data are presented in Table 3.

Kovalchuk A., Wang B., Li D., Rodriguez-Juarez R., Ilnytskyy S., Kovalchuk I, & Kovalchuk O. (2021). Fighting the storm: could novel anti-TNFα and anti-IL-6 C. sativa cultivars tame cytokine storm in COVID-19?. Aging (Albany NY), 13(2), 1571-1590.

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HPLC-DAD Analysis of Analytes

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HPLC-DAD analyses were performed on an Agilent 1200 HPLC system equipped with the following modules: G1312B BinPump SL, G13798 degasser, G1367D HiP ALS SL plus autosampler, a G1316B column compartment (set at 35 °C), and a G1315C diode array detector (Agilent, Waldbronn, Germany). Data acquisition rate was 80 Hz with the scan rate set between 210 – 400 nm (spectrum step 1 nm). The injection volume was 10 μL (0.1 mg/mL analyte solution). A Synergi Max-RP (250 × 4.6 mm, 4 μm) column from Phenomenex (Macclesfield, United Kingdom) was used and analytes were eluted under gradient conditions. Mobile phase A consisted of 25 mM triethylammonium phosphate (TEAP) buffer solution and mobile phase B comprised of 70% acetonitrile and 30% water containing 25 mM TEAP. The gradient elution commenced at 70% A and decreased to 5% within 5 min. This was then held until 12 min and returned to initial conditions by a 5 min post time.

Brandt S.D., Kavanagh P.V., Dowling G., Talbot B., Westphal F., Meyer M.R., Maurer H.H, & Halberstadt A.L. (2016). Analytical characterization of seventeen ring-substituted N,N-diallyltryptamines. Drug testing and analysis, 9(1), 115-126.

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