Radiolabeled 3h estrone 3 sulfate

Manufactured by PerkinElmer

Radiolabeled [3H]estrone-3-sulfate is a laboratory reagent used in research applications. It contains the naturally occurring estrogen estrone, which has been labeled with the radioactive isotope tritium (3H) and conjugated to a sulfate group. This product can be used as a tracer or standard in various analytical techniques, such as receptor binding assays or metabolic studies, to investigate the behavior and distribution of estrone-related compounds.

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Lab products found in correlation

Estradiol 17β glucuronide, by Merck Group (1 mentions) Mtses, by LGC (1 mentions) 3h estradiol 17β glucuronide, by PerkinElmer (1 mentions) Sulfo nhs ss biotin, by Thermo Fisher Scientific (1 mentions) Hek293 cell line, by American Type Culture Collection (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Proteintech (1 mentions)

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[3H]estrone-3-sulfate (15 citations)

2 protocols using radiolabeled 3h estrone 3 sulfate

Radiolabeled Estrone and Estradiol Glucuronide Assay

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Radiolabeled
[³H]estrone-3-sulfate
(57.3 Ci/mmol) and [³H]estradiol-17β-glucuronide
(46.9 Ci/mmol) were purchased from Perkin-Elmer (Boston, MA). Unlabeled
estrone-3-sulfate and estradiol-17β-glucuronide were purchased
from Sigma Aldrich (St. Louis, MO). Unlabeled valsartan, MTSES, and
[2-(trimethylammonium)ethyl]methanethiosulfonate bromide
(MTSET) were purchased from Toronto Research Chemicals, Inc. (North
York, Ontario, Canada). Sulfo-NHS-SS-biotin and maleimide-PEG₂-biotin were purchased from Pierce Biotechnology (Rockford,
IL). The polyclonal anti-His antibody was purchased from Covance (Princeton,
IL), whereas the monoclonal antibody to detect Na⁺/K⁺ ATPase α subunit was purchased from Abcam (Boston,
MA).

Ohnishi S., Hays A, & Hagenbuch B. (2014). Cysteine Scanning Mutagenesis of Transmembrane Domain 10 in Organic Anion Transporting Polypeptide 1B1. Biochemistry, 53(14), 2261-2270.

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Estrone-3-Sulfate Uptake Assay

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Radiolabeled [³H]estrone-3-sulfate was purchased from PerkinElmer (Waltham, MA). Unlabeled estrone-3-sulfate (E3S) was obtained from Sigma-Aldrich (St. Louis, MO). Fetal bovine serum (FBS), Dulbecco’s Modified Eagle’s medium (DMEM), and trypsin were from Hyclone (Logan, UT). Lipofectamine 2000 and Opti-MEM were purchased from Invitrogen (Carlsbad, CA). Sulfo-N-hydroxysuccinimide-SS-biotin, streptavidin-agarose beads, and the BCA protein assay kit were purchased from Pierce Chemical (Rockford, IL). Antibodies for detecting the 6-His tag and the Na⁺/K⁺-ATPase α subunit were purchased from Tiangen (Beijing, China) and Abcam (Boston, MA). Horseradish peroxidase-conjugated secondary antibodies were purchased from ProteinTech (Chicago, IL) and Sunshine Biotechnology (Nanjing, China). Immobilon Western blot detection kit was from Millipore (Billerica, MA). Human embryonic kidney (HEK293) cell line was from ATCC (Manassas, VA).

Yue M., Yang J., Jin M., Steiert B., Xiang Y., Zhang H., Hagenbuch B, & Gui C. (2019). Gly45 and Phe555 in Transmembrane Domains 1 and 10 are Critical for the Activation of OATP1B3 by Epigallocatechin Gallate. Journal of agricultural and food chemistry, 67(32), 9079-9087.

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