Alanine transaminase (ALT) release was determined in the blood using a point-of-care blood chemistry analyzer (Piccolo, Abaxis, Union City, CA). Levels of C-reactive protein (CRP), TNF- α, IL-1β, IFN-γ and IL-12 were determined in the blood using a multiplex bead array (Eve Technologies, Calgary, Canada). Absolute concentration of adenosine triphosphate (ATP) was measured in liver samples using a luminescence-based assay (Cell Viability Kit; Biovision). Nucleotides were extracted from ~1 mg of homogenized tissue, after which peak luminescence was determined. ATP concentrations were normalized to tissue protein using a BCA protein assay (Thermo Fisher Scientific, Waltham, MA).
Multiplex bead array
Manufactured by Eve Technologies
Sourced in Canada
The Multiplex bead array is a versatile lab equipment that enables the simultaneous detection and quantification of multiple analytes in a single sample. It utilizes color-coded microspheres, each coated with specific capture antibodies, to provide a high-throughput and multiplexed analysis platform.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using multiplex bead array
1
Biomarker Assessment in Liver Samples
2
Multiplex Analysis of Inflammatory Markers
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