THP-1 cells were seeded at 5 × 104 cells per well in 24-well culture plates for 24 h. After incubation with starvation medium (0.5% FBS) for 24 h, cells were intervened with either human cortisol (H-CORT., 10− 9 to 10− 5 mol/L, Sigma-Aldrich, HA-8880) or PBS for 48 h. Positive control group was intervened with lipopolysaccharides (LPS, 100 ng/ml, Sigma-Aldrich, L6529). Supernatant was frozen at − 80 °C until being used for matrix metalloprotein-2 (MMP-2, Raybiotech, ELH-MMP2), tissue inhibitor of metalloproteinase-2 (TIMP-2, Raybiotech, ELH-TIMP2) and tumor necrosis factor-α (TNF-α, Raybiotech, ELH-TNFα) detection.
Elh timp2
Manufactured by RayBiotech
Sourced in United States
The ELH-TIMP2 is a laboratory equipment product manufactured by RayBiotech. It is designed to detect and quantify the levels of TIMP2 (Tissue Inhibitor of Metalloproteinase 2) in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using elh timp2
1
Cortisol Modulation of THP-1 Cells
2
Quantification of Inflammatory Markers in Intervertebral Disc Culture
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The concentration of PGE 2 (K051-H5, Arbor Assays, Ann Arbor, MI, USA), bIL-6 (MBS9141101, MyBioSource San Diego, CA, USA), hIL-6 (430507, BioLegend), hIL-1β (DLB50, R&D Systems), hIL-1ra (BRA00B, R&D Systems), hCFH (ab137975, Abcam), hTIMP-1 (ELH-TIMP1, RayBiotech, Peachtree Corners, GA, USA) and hTIMP-2 (ELH-TIMP2, RayBiotech) was determined by ELISA in the supernatants at days 11 and 16 of organ culture.
DNA and protein quantification in the AF tissue AF tissues were digested overnight at 56 °C using 0.5 mg/mL proteinase K (P6556, Sigma-Aldrich) solution for DNA and sGAG quantification. DNA content was determined using the Quant-iT PicoGreen dsDNA assay kit (P7589, Invitrogen). sGAG content was determined using the Blyscan assay kit (B1000, Biocolor, Carrickfergus, UK). AF tissues were digested for soluble collagen and elastin quantification according to the Sircol (S1000, Biocolor) and Fastin (F2000, Biocolor) assay kits, respectively.
DNA and protein quantification in the AF tissue AF tissues were digested overnight at 56 °C using 0.5 mg/mL proteinase K (P6556, Sigma-Aldrich) solution for DNA and sGAG quantification. DNA content was determined using the Quant-iT PicoGreen dsDNA assay kit (P7589, Invitrogen). sGAG content was determined using the Blyscan assay kit (B1000, Biocolor, Carrickfergus, UK). AF tissues were digested for soluble collagen and elastin quantification according to the Sircol (S1000, Biocolor) and Fastin (F2000, Biocolor) assay kits, respectively.
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