For light microscopy of living cells, in vitro haptomonad-like cells attached to a piece of a gridded glass coverslip were washed twice in DMEM, incubated in DMEM with Hoechst 33342 (1 µg/ml) for 5 min and then washed twice in DMEM. Coverslip pieces were mounted onto another glass coverslip and then onto a glass slide, with the cell attachment side facing up. Attached cells were imaged using a Zeiss ImagerZ2 microscope with 63×objective and a Hamamatsu Flash 4 camera.
Imagerz2
Manufactured by Hamamatsu Photonics
The ImagerZ2 is a lab equipment product manufactured by Hamamatsu Photonics. It is a high-performance imaging device designed for various scientific and research applications. The ImagerZ2 is capable of capturing high-quality images and data, but a detailed description of its core function is not available at this time.
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Lab products found in correlation
2 protocols using imagerz2
1
Live Cell Microscopy Protocol
2
Fluorescence Imaging of Tagged Proteins
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Cells were immobilized on a 1% agarose gel pad and viewed with a Zeiss Axio Imager Z2 epifluorescence microscope equipped with a Hamamatsu Orca-Flash 4.0 sCMOS camera and a Plan-Apochromat 100x/1.46 Oil Ph3 objective. Zen Blue software was used for image capture and quantification. For fluorescence imaging, mChy was observed by excitation at 587 nm and emission at 610 nm, GFP by excitation at 488 nm and emission at 509 nm, and YFP by excitation at 508 nm and emission at 524 nm. Exposure times for imaging mChy, GFP and YFP tagged proteins were 500 ms, 500 ms and 1000 ms respectively. All microscopy was performed at 1000X magnification.
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