Paraffin-embedded BM sections were incubated with the following: rabbit anti-PDCD4 (1:200; abcam), mouse anti-CD34 (1:5; Dako), and rabbit anti-4-hydroxy-2-nonenal (4-HNE) (1:500; Bioss). Nuclei were counterstained with DAPI (1 μg/mL) (Sigma-Aldrich). Images were acquired with a fluorescent microscope (DM6 B; Leica Microsystems) at 63× magnification and analyzed using Fiji software. Cultured HUVECs were fixed with 4% paraformaldehyde (PFA) (Electron Microscopy Science), permeabilized with 0.3% triton (Sigma-Aldrich), and stained with rabbit anti-PDCD4 (1:200). Nuclei were counterstained with DAPI (1 μg/mL) (Sigma-Aldrich). Microphotographs were captured using a Zeiss microscope equipped with digital image processing software (AxioVision Imaging System).
Mouse anti cd34
Manufactured by Agilent Technologies
Sourced in Denmark
Mouse anti-CD34 is a laboratory reagent used for the detection and identification of CD34 antigen. CD34 is a cell surface glycoprotein expressed on hematopoietic stem and progenitor cells. This product can be used in various applications, such as flow cytometry and immunohistochemistry, to study the expression of CD34 in cells and tissues.
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2 protocols using mouse anti cd34
1
Immunofluorescence Analysis of PDCD4, CD34, and 4-HNE
2
Double Immunohistochemistry for Vascular and Renal Analysis
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Double immunohistochemical staining with mouse anti-CD34 (Dako, Glostrup, Denmark) and anti-SMA (Dako) was performed to identify endothelial cells and smooth muscle cells of the arterial media (Figure 1 A). The PAS stain was then applied to the sections to identify the tubular basement membranes, Bowman’s capsules, sclerotic glomeruli, and interstitial fibrosis (Figure 2 F,G, Figure 3 A,G, Figure 4 F,G, Figure 5 C,F,G and Figure 6 G). Details of the tissue preparation are described elsewhere [7 (link),8 (link),9 (link),10 (link)]. Each stained specimen was digitized using virtual slide microscopy (NanoZoomer RS™; Hamamatsu Photonics, Hamamatsu, Japan), using a 40× optical lens.
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