Esi q tof ms ms

Manufactured by Thermo Fisher Scientific

Sourced in United States

The ESI-Q-TOF MS/MS is a high-resolution mass spectrometry instrument designed for accurate mass measurements and detailed structural analysis of small molecules and biomolecules. It utilizes electrospray ionization (ESI) for sample introduction and a quadrupole-time-of-flight (Q-TOF) mass analyzer configuration.

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Lab products found in correlation

Mascot, by Matrix Science (1 mentions) Mascot program, by Matrix Science (1 mentions)

2 protocols using esi q tof ms ms

Proteome Analysis of Diploid Oysters

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Proteins extracted from diploid oysters were resolved by 8% sodium dodecyl
sulfate-polyacrylamide gel electrophoresis and identified by tandem mass
spectrometry (MS/MS) and electrospray ionization quadrupole time-of-flight MS/MS
(ESI-Q-TOF MS/MS, ABI), as described previously (Choi et al., 2015 (link)). Proteins were identified using the
NCBI (https://www. ncbi.nlm.nih.gov) and UniProt Knowledgebase
(http://www.uniprot.org/uniprot) databases and MASCOT (Matrix
Science, London, UK) and FASTA software.

Park S.J, & Choi Y.H. (2022). Relationship between Condition Index Values and Expression Levels of Gene and Protein in the Adductor Muscle of Diploid and Triploid Oysters Crassostrea gigas. Development & Reproduction, 26(4), 165-174.

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Proteomic Analysis of Gonadal Proteins

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We investigated protein expression in gonads using 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Specific proteins were confirmed using tandem mass spectrometry (MS/MS) and electrospray ionization quadrupole time-of-flight MS/MS (ESI-Q-TOF MS/MS, ABI, USA) as previously described (Choi et al. 2015) . Proteins were identified via the National Center for Biotechnology Information (NCBI; https://www.ncbi.nlm.nih.gov) and UniProt Knowledgebase (http://www.uniprot.org/uniprot) databases using the MASCOT program (Matrixscience, London, UK).

Choi Y.H., & Choi Y. (2020). Role of the insulin-like growth factor system in gonad sexual maturation in Pacific oyster Crassostrea gigas. Fisheries and aquatic sciences, 23(1).

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