Anti ctgf

Manufactured by Sangon

Sourced in China

Anti-CTGF is a laboratory reagent used for the detection and quantification of connective tissue growth factor (CTGF) in various biological samples. It is a specific antibody that binds to CTGF, a key regulator of cellular processes involved in tissue repair and remodeling.

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Lab products found in correlation

4 protocols using anti ctgf

Antibody Panel for Cellular Signaling Analysis

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Antibodies used in this study include anti-β-catenin (BD Biosciences, Cat# 610153, RRID: AB_397554), anti-KI67 (Abcam, Cat# ab15580, RRID: AB_443209), anti-YAP (Cell Signaling Technology, Cat# 14074, RRID: AB_2650491), anti-CTGF (Sangon Biotech, Cat# D260212, Shanghai, China), anti-CYR61 (Cell Signaling Technology, Cat# 39382, RRID: AB_2799154), anti-ANKRD1 (Sangon Biotech, Cat# D121628, RRID: AB_2819216), anti-CCNA1 (Sangon biotech, Cat# D220507, RRID: AB_2819214), anti-CCND1 (Sangon biotech, Cat# D220509), anti-CCNE1 (Sangon biotech, Cat# D151593), anti-p-YAP Ser127 (Cell Signaling Technology, Cat# 13008, RRID: AB_2650553), anti-β-actin (Cell Signaling Technology, Cat# 8457, RRID: AB_10950489), anti-lamin B (Cell Signaling Technology, Cat# 13435, RRID: AB_2737428), anti-c-Myc (Cell Signaling Technology, Cat# 9402, RRID: AB_2151827), anti-CTNNB1 (Sangon biotech, Cat# D199519), anti-MET (Sangon biotech, Cat# D160981) and anti-FOXM1 (Proteintech, Cat# 13147-1-AP, RRID: AB_2106213, Rosemont, IL, USA).

Gao Y., Fan S., Li H., Jiang Y., Yao X., Zhu S., Yang X., Wang R., Tian J., Gonzalez F.J., Huang M, & Bi H. (2020). Constitutive androstane receptor induced-hepatomegaly and liver regeneration is partially via yes-associated protein activation. Acta Pharmaceutica Sinica. B, 11(3), 727-737.

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Pregnenolone 16α-carbonitrile Metabolic Pathway

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Pregnenolone 16α-carbonitrile (PCN) with 98% purity (Cat# C3884) was purchased from ApexBio Technology (Houston, USA). Additionally, corn oil (Cat# C116025) and vancomycin (Cat# C10791097) were purchased from Aladdin Company (Shanghai, China).
Neomycin sulfate (Cat#N814740), metronidazole (Cat# M813526) and ampicillin (Cat# C116025) were obtained from Macklin (Shanghai, China). Rabbit polyclonal anti-CYP3A11 (Cat# A13484) antibody and anti-CYP2B10 (Cat# A1463) antibody were purchased from Abclonal (Wuhan, China). Rabbit monoclonal anti-β-actin (Cat# AC038) antibody was purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit polyclonal anti-ANKRD1 (Cat# D121628), anti-CYR61 (Cat# D322190) and anti-CTGF (Cat# D260212) were all provided by Sangon Biotechnology (Shanghai, China).

Wu T., Li L., Zhou W., Bi G., Jiang X., Guo M., Yang X., Fang J., Pang J., Fan S, & Bi H. (2024). Gut Microbiota Affects Mouse Pregnane X Receptor Agonist Pregnenolone 16α-Carbonitrile-Induced Hepatomegaly by Regulating Pregnane X Receptor and Yes-Associated Protein Activation. Drug metabolism and disposition: the biological fate of chemicals, 52(7).

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Signaling Pathway Antibody Panel

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Anti-YAP (Cat# 14074S, RRID: AB_2650491), anti-Phospho-YAP (Ser127) (Cat# 13008S, RRID: AB_2650553), anti-Cyclin D1 (Cat# 2922S, RRID: AB_2228523), anti-Cyclin D1 (Cat# 55506S, RRID: AB_2827374), anti-Cyclin E1 (Cat# 20808, RRID: AB_2783554), anti-β-actin (Cat# 4970S, RRID, AB_2223172), anti-Lamin B1 (Cat# 13435S, RRID: AB_2737428), anti-GAPDH (Cat# 2118S, RRID: AB_561053), anti-rabbit, IgG HRP-linked antibody (Cat# 7074S, RRID: AB_2099233), anti-mouse IgG, HRP-linked antibody (Cat# 7076S, RRID: AB_330924), signalstain® boost IHC detection reagent (HRP, rabbit) (Cat# 8114S, RRID: AB_10544930), signalstain® boost IHC detection reagent (HRP, mouse) (Cat# 8125S, RRID: AB_10547893) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-CCNA1 (Cat# D220507, RRID: AB_2819214), anti-CYR61 (Cat# D122190, RRID: AB_2819221), anti-ANKRD1 (Cat# D121628, RRID: AB_2819216) and anti-CTGF (Cat# D160212, RRID: AB_2819217) were purchased from Sangon Biotech (Shanghai, China). Anti-β-catenin (Cat# 610153, RRID: AB_397554) was purchased from BD Biosciences (Franklin Lakes, NJ, USA). Anti-caspase-3 (Cat# sc-56053, RRID: AB_781826) was purchased from Santa Cruz Biotechnology, Inc. (Carpinteria, CA, USA).

Li X., Fan S., Cai C., Gao Y., Wang X., Zhang Y., Liang H., Li H., Yang J., Huang M, & Bi H. (2022). YAP regulates the liver size during the fasting-refeeding transition in mice. Acta Pharmaceutica Sinica. B, 13(4), 1588-1599.

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Protein Expression Analysis by Western Blot

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The total protein was extracted from cells using a cold RIPA lysis buffer. Protein samples (20 μg) were separated by 12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Millipore, Billerica, USA). The membranes were blocked in a QuickBlock™ blocking buffer (Beyotime) for 30 min and then incubated with primary antibodies including anti-p21 (1:800; ProteinTech), anti-α-SMA (1:1000; ProteinTech), anti-CTGF (1:800; Sangon Biotech, Shanghai, China), anti-CDK1(Tyr15) (1:1000; Cell Signaling Technology), anti-CDC25C(Ser216) (1:1000; Cell Signaling Technology), and anti-GAPDH (1:5000; ProteinTech) at 4°C overnight. Subsequently, the membranes were incubated with the corresponding HRP-conjugated secondary antibodies at room temperature for 1 h. Finally, protein bands were visualized using a chemiluminescence kit (Fdbio Science, Hangzhou, China), and the images were analyzed using ImageJ software. GAPDH was used as the loading control.

Lu W., Ren S., Dong W., Li X., Zheng Z., Jia Y, & Xue Y. (2022). Albumin-induced premature senescence in human renal proximal tubular cells and its relationship with intercellular fibrosis: Effect of premature senescence stimulated by albumin on fibrosis in HK-2 cells. Acta Biochimica et Biophysica Sinica, 54(7), 893-903.

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