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Hrp conjugated goat anti mouse and anti rabbit iggs

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HRP-conjugated goat anti-mouse and anti-rabbit IgGs are secondary antibodies used in immunoassays. They bind to the primary antibody that recognizes the target antigen and are conjugated to the enzyme Horseradish Peroxidase (HRP), which can be detected and quantified.

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Lab products found in correlation

Gtx117624, by Abcam (2 mentions) Gtx111677s, by GeneTex (2 mentions) Fluorchem hd2 apparatus, by Bio-Techne (2 mentions) Trans blot sd semi dry transfer cell apparatus, by Bio-Rad (2 mentions)

Close spelling variants of this product

HRP-conjugated goat anti-mouse IgG (13 citations) Goat anti-mouse IgG-HRP (12 citations) Goat anti-rabbit IgG-HRP (9 citations) HRP-conjugated goat anti-rabbit IgG (7 citations) HRP-conjugated anti-mouse IgG (7 citations) Goat anti-mouse IgG (6 citations) Goat anti-rabbit IgG (4 citations)

2 protocols using hrp conjugated goat anti mouse and anti rabbit iggs

1

Western Blot Analysis of Telomeric Proteins

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Total proteins prepared in 2X Laemmli buffer were separated in 8-10% polyacrylamide gels and transferred to nitrocellulose membranes (Maine Manufacturing LLC) using a Trans-Blot SD Semi-Dry Transfer Cell apparatus (Bio-Rad). Western blots were performed according to standard procedures using the following primary antibodies: a mouse monoclonal anti-TRF2 (Millipore, 05-521, dilution 1:2000), a rabbit polyclonal anti-TRF1 raised against full-length recombinant TRF1 (kind gift from Jan Karlseder, 1:2000), a rabbit polyclonal anti-Tankyrase 1 (kind gift from Susan Smith, 1:5000), a rabbit polyclonal anti-RNaseH1 (GeneTex, GTX117624, 1:1000), a mouse monoclonal anti-hbetaActin (Abcam, ab8224, 1:10000), and a rabbit polyclonal anti-LaminA (GeneTex, GTX111677S, 1:5000). Secondary antibodies were HRP-conjugated goat anti-mouse and anti-rabbit IgGs (Bethyl Laboratories, 1:5000). Signals were acquired using a FluorChem HD2 apparatus (Alpha Innotech).
Lee Y.W., Arora R., Wischnewski H, & Azzalin C.M. (2018). TRF1 participates in chromosome end protection by averting TRF2-dependent telomeric R-loops. Nature structural & molecular biology, 25(2), 147-153.
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2

Western Blot Analysis of Telomeric Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total proteins prepared in 2X Laemmli buffer were separated in 8-10% polyacrylamide gels and transferred to nitrocellulose membranes (Maine Manufacturing LLC) using a Trans-Blot SD Semi-Dry Transfer Cell apparatus (Bio-Rad). Western blots were performed according to standard procedures using the following primary antibodies: a mouse monoclonal anti-TRF2 (Millipore, 05-521, dilution 1:2000), a rabbit polyclonal anti-TRF1 raised against full-length recombinant TRF1 (kind gift from Jan Karlseder, 1:2000), a rabbit polyclonal anti-Tankyrase 1 (kind gift from Susan Smith, 1:5000), a rabbit polyclonal anti-RNaseH1 (GeneTex, GTX117624, 1:1000), a mouse monoclonal anti-hbetaActin (Abcam, ab8224, 1:10000), and a rabbit polyclonal anti-LaminA (GeneTex, GTX111677S, 1:5000). Secondary antibodies were HRP-conjugated goat anti-mouse and anti-rabbit IgGs (Bethyl Laboratories, 1:5000). Signals were acquired using a FluorChem HD2 apparatus (Alpha Innotech).
Lee Y.W., Arora R., Wischnewski H, & Azzalin C.M. (2018). TRF1 participates in chromosome end protection by averting TRF2-dependent telomeric R-loops. Nature structural & molecular biology, 25(2), 147-153.
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