Rabbit anti gapdh

Manufactured by Antgene

Sourced in United States

Rabbit anti-GAPDH is a primary antibody used in various laboratory techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry. It specifically recognizes and binds to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein, which is a widely expressed and highly conserved enzyme involved in glycolysis.

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Lab products found in correlation

Anti sox2, by Proteintech (1 mentions) Anti dnmt3a, by Cell Signaling Technology (1 mentions) Anti dnmt3b, by Cell Signaling Technology (1 mentions) Anti oct4, by Proteintech (1 mentions) Rabbit anti dnmt1, by Cell Signaling Technology (1 mentions) Ripa lysis buffer, by Wuhan Servicebio Technology (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Ecl system, by Bio-Rad (1 mentions)

Spelling variants of this product

GAPDH (4 citations) Anti-GAPDH (2 citations)

2 protocols using rabbit anti gapdh

Protein Expression Analysis of Stem Cells

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Cells were lysed using lysis buffer and extracted for 20 min on ice. Whole-cell extractprotein (30 μg) was subjected to electrophoresis on 10% SDS-PAGE gel and transferred to nitrocellulose membranes. Then membranes were incubated with the following primary antibodies: rabbit anti-Nanog, anti-Oct-4, anti-Sox-2 (dilution 1:1000, Proteintech, Rosemont, USA), rabbit anti-DNMT1, anti-DNMT3A, anti-DNMT3B (dilution 1:1000, Cell Signaling Technology, Danvers, MA, USA), and rabbit anti-GAPDH (dilution 1:2000, Antgene, Wuhan, China).

Ai L., Mu S., Sun C., Fan F., Yan H., Qin Y., Cui G., Wang Y., Guo T., Mei H., Wang H, & Hu Y. (2019). Myeloid-derived suppressor cells endow stem-like qualities to multiple myeloma cells by inducing piRNA-823 expression and DNMT3B activation. Molecular Cancer, 18, 88.

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Cellular Protein Expression Analysis

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Cells were lysed for 30 minutes in ice-cold RIPA lysis buffer (Servicebio Inc, Wuhan, China) and a protease inhibitor cocktail (Roche). The following primary antibodies used: rabbit anti-GAPDH (1:2000, Antgene, Wuhan, China), rabbit anti-HMGA2 (1:200, A12743, ABclonal), rabbit anti-Bcl-2 (1:1000, 12789-1-AP, Proteintech), and rabbit anti-Caspase 3 (1:1000, A11953, ABclonal). Protein was detected with horseradish peroxidase-conjugated anti-rabbit IG secondary antibody using the ECL system (Bio-Rad).

Wang L., Shen H., Zhu D., Feng B., Yu L., Tian X., Ren C., Gao C., Li X., Ma D., Hu Z, & Wang H. (2018). Increased high mobility group A 2 expression promotes transition of cervical intraepithelial neoplasm into cervical cancer. Oncotarget, 9(8), 7891-7901.

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