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Ito slides

Manufactured by Nanion Technologies
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ITO slides are transparent conductive glass substrates used in various laboratory and research applications. They are composed of a thin layer of indium tin oxide (ITO) deposited onto a glass surface, providing electrical conductivity while maintaining optical transparency.

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Lab products found in correlation

Vesicle prep pro, by Nanion Technologies (2 mentions) Rh pe, by Avanti Polar Lipids (2 mentions)

2 protocols using ito slides

1

Formation of Giant Unilamellar Vesicles

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Chloroform stock solutions of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, Avanti Polar Lipids), 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS, Avanti Polar Lipids), and cholesterol (Sigma) were mixed at a final lipid concentration of 0.25 mM and labeled with 0.1% Rhodamine-PE (RH-PE, Avanti Polar Lipids). GUVs were grown on ITO slides (Nanion Technologies) by gently spreading 30 μL of the lipid solution and evaporating the solvent by an argon stream, followed by desiccation under a mild vacuum for at least 2 h. GUVs were then grown by electroformation in 275 mL of 300 mM sucrose solution using a Vesicle Prep Pro instrument (Nanion Technologies). First, the electroformation voltage was increased stepwise to 3 V, 15 Hz) and applied at 55 °C for 2 h, followed by a slow decrease of voltage and frequency (see SI Appendix B, Figure S1).
Shendrik P., Golani G., Dharan R., Schwarz U.S, & Sorkin R. (2023). Membrane Tension Inhibits Lipid Mixing by Increasing the Hemifusion Stalk Energy. ACS Nano, 17(19), 18942-18951.
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2

Formation of Giant Unilamellar Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chloroform stock solutions of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, Avanti Polar Lipids), 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS, Avanti Polar Lipids), and cholesterol (Sigma) were mixed at a final lipid concentration of 0.25 mM and labeled with 0.1% Rhodamine-PE (RH-PE, Avanti Polar Lipids). GUVs were grown on ITO slides (Nanion Technologies) by gently spreading 30 μL of the lipid solution and evaporating the solvent by an argon stream, followed by desiccation under a mild vacuum for at least 2 h. GUVs were then grown by electroformation in 275 mL of 300 mM sucrose solution using a Vesicle Prep Pro instrument (Nanion Technologies). First, the electroformation voltage was increased stepwise to 3 V, 15 Hz) and applied at 55 °C for 2 h, followed by a slow decrease of voltage and frequency (see SI Appendix B, Figure S1).
Shendrik P., Golani G., Dharan R., Schwarz U.S, & Sorkin R. (2023). Membrane Tension Inhibits Lipid Mixing by Increasing the Hemifusion Stalk Energy. ACS Nano, 17(19), 18942-18951.
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