Polycaprolactone (PCL, MW 70-90 kDa) and polyethylene glycol-diamine (PEG-diamine, average MW 3,000) were purchased from Sigma Aldrich (St. Louis, MO). Sodium Chloride (NaCl) was procured from Fisher Scientific (Pittsburgh, PA). 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP) was obtained from Oakwood Products, while dichloromethane (DCM) came from (Pharmco-AAPER, Brookfield, CT). Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Grand Island, NY), RPMI medium, PC-12 (PC-12 (ATCC CRL-1721, Manassas, VA), laminin (L4544, Sigma Aldrich), Laminin Antibody conjugated Alexa Fluor 488 (NB300-144AF488, Novusbio), laminin ELISA kit (abl 19599; Abcam, Cambridge, MA), Anti-Neurofilament 200 antibody (N4142, Sigma Aldrich), goat anti-rabbit IgG (H+L) with fluorescein isothiocyanate 1 (FITC) (111-095-045, Jackson Immunoresearch Laboratories, Philadelphia, PA) were used in this study.
Polyethylene glycol diamine
Manufactured by Merck Group
Polyethylene glycol-diamine is a synthetic organic compound commonly used as a linker or spacer in various biochemical and biomedical applications. It serves as a versatile tool for conjugating molecules, facilitating the connection between different compounds or materials.
Automatically generated - may contain errors
Lab products found in correlation
Anti neurofilament 200 antibody, by Merck Group (1 mentions)
Polycaprolactone, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions)
2 hydroxy 2 methylpropiophenone, by Merck Group (1 mentions)
Nile red, by Merck Group (1 mentions)
Rhodamine 6g, by Merck Group (1 mentions)
1 6 hexanediol diacrylate hdoda, by Merck Group (1 mentions)
No 1.5h, by Marienfeld (1 mentions)
2 protocols using polyethylene glycol diamine
1
Fabrication of Laminin-Enriched PCL-PEG Scaffolds
2
Photocurable Nanodroplet Formation on Functionalized Surfaces
Check if the same lab product or an alternative is used in the 5 most similar protocols
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1,6-Hexanediol diacrylate (HDODA)
(80%, Sigma) and lauryl methacrylate (LMA) (97%, Sigma) were utilized
as monomers. The droplets of monomers were cured through the incorporation
of photoinitiator, 2-hydroxy-2-methylpropiophenone (97%, Sigma). Isopropanol
(AR, Sigma) was used to clean the solid substrates, syringes, and
fluid cell. Ethanol (AR, Chem-supply) and water (Milli-Q) were utilized
as the good and poor solvent, respectively, for the solvent exchange
to produce surface nanodroplets. For fluorescent studies, the solution
was stained by either Nile red (Sigma) and Rhodamine 6G (Sigma). Silver
nitrate (99% Chem-supply) and trisodium citrate (99%, Chem-supply)
were nanoparticle precursors. Poly(ethylene glycol)diamine (Mn = 3000, Sigma) was utilized to functionalize
the polymeric lens with amine groups. All reagents were used as received.
Cover glass (ϕ = 42 mm, No. 1, Proscitech or 26 × 60 mm,
No. 1.5H, Marienfeld) were used as substrates. No unexpected or unusually
high safety hazards were encountered.
(80%, Sigma) and lauryl methacrylate (LMA) (97%, Sigma) were utilized
as monomers. The droplets of monomers were cured through the incorporation
of photoinitiator, 2-hydroxy-2-methylpropiophenone (97%, Sigma). Isopropanol
(AR, Sigma) was used to clean the solid substrates, syringes, and
fluid cell. Ethanol (AR, Chem-supply) and water (Milli-Q) were utilized
as the good and poor solvent, respectively, for the solvent exchange
to produce surface nanodroplets. For fluorescent studies, the solution
was stained by either Nile red (Sigma) and Rhodamine 6G (Sigma). Silver
nitrate (99% Chem-supply) and trisodium citrate (99%, Chem-supply)
were nanoparticle precursors. Poly(ethylene glycol)diamine (Mn = 3000, Sigma) was utilized to functionalize
the polymeric lens with amine groups. All reagents were used as received.
Cover glass (ϕ = 42 mm, No. 1, Proscitech or 26 × 60 mm,
No. 1.5H, Marienfeld) were used as substrates. No unexpected or unusually
high safety hazards were encountered.
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