Neem oil was purchased by Neem Italia (Moniga del Garda (BS), Italy) and is characterised by an ECOCERT certificate (Biocert Italia IT013BC041 – ICEA 264BC001). Tween 20 (Tw20), Hepes salt {N-(2-hydroxyethyl) piperazine-N-(2-ethanesulphonic acid)} and pyrene were Sigma-Aldrich products (Sigma-Aldrich, Milan, Italy). Distilled water (Ecological Product System s.r.l., Rome, Italy) was used for all experiments. All other products and reagents were of analytical grade.
Tween 20 tw20
Manufactured by Merck Group
Sourced in Italy
Tween 20 (Tw20) is a non-ionic detergent commonly used in various laboratory applications. It is a polyoxyethylene sorbitan monolaurate with the chemical formula C58H114O26. Tween 20 is a clear, viscous liquid used to solubilize and stabilize proteins, facilitate cell lysis, and enhance the performance of immunoassays and other biochemical techniques.
Automatically generated - may contain errors
Lab products found in correlation
Hepes salt, by Merck Group (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Heat inactivated fbs, by Thermo Fisher Scientific (1 mentions)
Mtt assay, by Promega (1 mentions)
Sephadex g 75, by Merck Group (1 mentions)
Nh4 2so4, by Merck Group (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Trypsin edta 1 solution, by Thermo Fisher Scientific (1 mentions)
Mcf 7 and mda mb 468, by American Type Culture Collection (1 mentions)
Whatman, by Cytiva (1 mentions)
Polyvinylpyrrolidone pvp, by Merck Group (1 mentions)
Lipofectin, by Thermo Fisher Scientific (1 mentions)
Pluronic f127 pf127, by Merck Group (1 mentions)
18 mer splice switching on, by GE Healthcare (1 mentions)
Tween 80 tw 80, by Merck Group (1 mentions)
Pluronic f 68 pf68, by Merck Group (1 mentions)
2 hydroxypropyl β cyclodextrin hpβcd, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Phosphoric acid, by Merck Group (1 mentions)
4 protocols using tween 20 tw20
1
Neem Oil Characterization and Application
2
Liposomal Doxorubicin Formulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tween 20 (Tw20), Hepes salt, [N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic
acid)], polycarbonate Whatman nucleopore tracketched membranes (cut-off
0.1 μm), DPPG, Sephadex G75, (NH4)2SO4, and H2CO3 were purchased from Sigma-Aldrich
(Sigma-Aldrich SRL, Milan, Italy). Chol and sorbitan monolaurate (Span20)
were obtained from Acros Organics products (Acros Organics BVBA Geel,
Belgium). Cellulose acetate membrane (cut-off 8 kDa) was obtained
from Prodotti Gianni S.p.A. (Milan, Italy). MTT assay was obtained
from Promega (Madison, WI, USA). DOX was obtained by D.B.A. (Milan,
Italy). MCF-7 and MDA MB 468 were obtained from American Type Culture
Collection (ATCC, LGC Standards, Teddington, UK). High-glucose Dulbecco’s
modified Eagle’s minimal essential medium (DMEM), Roswell Park
Memorial Institute Medium (RPMI-1640), heat-inactivated FBS, trypsin–EDTA
(1×) solution, and penicillin–streptomycin solution were
obtained from Gibco (Invitrogen Corporation, Giuliano Milanese, Milan,
Italy). Lissamine rhodamine B 1,2 dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (rhodamine B-DHPE)
was purchased from Invitrogen (Life Technologies Corporation, Grand
Island, NY, USA). All other chemical reagents were obtained from Sigma-Aldrich
(Milan, Italy) or Thermo Fisher Scientific (Waltham, MA USA) and are
used without further purification.
acid)], polycarbonate Whatman nucleopore tracketched membranes (cut-off
0.1 μm), DPPG, Sephadex G75, (NH4)2SO4, and H2CO3 were purchased from Sigma-Aldrich
(Sigma-Aldrich SRL, Milan, Italy). Chol and sorbitan monolaurate (Span20)
were obtained from Acros Organics products (Acros Organics BVBA Geel,
Belgium). Cellulose acetate membrane (cut-off 8 kDa) was obtained
from Prodotti Gianni S.p.A. (Milan, Italy). MTT assay was obtained
from Promega (Madison, WI, USA). DOX was obtained by D.B.A. (Milan,
Italy). MCF-7 and MDA MB 468 were obtained from American Type Culture
Collection (ATCC, LGC Standards, Teddington, UK). High-glucose Dulbecco’s
modified Eagle’s minimal essential medium (DMEM), Roswell Park
Memorial Institute Medium (RPMI-1640), heat-inactivated FBS, trypsin–EDTA
(1×) solution, and penicillin–streptomycin solution were
obtained from Gibco (Invitrogen Corporation, Giuliano Milanese, Milan,
Italy). Lissamine rhodamine B 1,2 dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (rhodamine B-DHPE)
was purchased from Invitrogen (Life Technologies Corporation, Grand
Island, NY, USA). All other chemical reagents were obtained from Sigma-Aldrich
(Milan, Italy) or Thermo Fisher Scientific (Waltham, MA USA) and are
used without further purification.
3
Splice-switching ON and Gene Delivery Vectors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 18-mer splice-switching ON with the sequence (5′-CCUCUUACCUCAGUUACA) was synthesized at GE Healthcare. The ON had a fully modified phosphorothioate backbone and 2′-O-methyl modified ribose sugar units. 187.15-5LNA (5′-aGacTuaCcaCuuCc) was used for the BTK gene cell models [21 (link)]. The G2-RR peptide dendrimer (Figure 1 ) used in the study was synthesized using solid phase synthesis while applying Fmoc/Boc protection strategy, as reported previously [17 (link)]. Lipofectin (a liposomal formulation of a 1:1 (w/w) ratio of DOTMA (N-[1-(2,3-dioleyloxy)- propyl]-N,N,N-trimethylammonium chloride) and DOPE (dioleoyl phosphotidylethanolamine)), and Lipofectamine 2000 (L2000) were both purchased from Invitrogen (Stockholm, Sweden). Polyvinylpyrrolidone “PVP” (average molecular weights: 10, 40, and 55 kDa); Polyvinylalcohol “PVA” (average molecular weights: 18, and 40 kDa); Pluronic F 68 “PF68”; Pluronic F 127 “PF127”; Tween 80 “TW80”; Tween 20 “TW20”; (2-Hydroxypropyl)-β-cyclodextrin “HP-β-CD” and all other sugars were bought from Sigma–Aldrich (Stockholm, Sweden).
4
Meloxicam-loaded Hydrophilic Matrices Fabrication
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Meloxicam (Techno Drugs & Intermediates Ltd. Mumbai, India), hydroxypropyl methylcellulose (HPMCE5 , Ethocel E5) with a viscosity of 5 mPa • s, (Dow Chemical Co., Midland, USA) and HPMC15000 (Metolose 90SH) with a viscosity of 15000 mPa • s, (Shin-Etsu Chemical Co., Ltd. Tokyo, Japan), propylene glycol (PG) (Scharlau Chemie, Barcelona, Spain), Tween 20 (Tw20) (Sigma Aldrich Co., France), ethanol (Chemical Company, Romania), acetonitrile (Merk, Germany), methanol (Merk, Germany) as HPLC solvents with HPLC analytical grade; ultrapure water; potassium phosphate KH2PO4 (Merk, Germany) and phosphoric acid (Merk, Germany). Preparation of hydrophilic matrices Three formulations have been prepared, using two types of hydrophilic polymers (Table I). The used solvent evaporation method was described previously [2] , but briefly MX was added in ethanol and propylene glycol under continuous stirring. After that a mixture of Tween 20 and ultrapure water was added and stirring was continued for two hours to disperse the hydrophilic polymer. This dispersion was sonicated in an ultrasound bath for 30 minutes and casted into Petri dishes (9.8 cm diameter). The matrices were dried for 24 h at 40°C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!