Deuterated chloroform chcl3 d

All of the reagents including α-glucosidase, dimethyl sulfoxide (DMSO), potassium hydroxide (KOH), phosphate buffer, p-nitrophenyl-glucopyranoside (p-NPG), acarbose, phosphoric acid (H₃PO₄), human salivary α-amylase, 2-chloro-4-nitrophenyl-maltotrioside (CNP-G3), tris-HCl, porcine pancreas lipase, p-nitrophenyl butyrate (p-NPB), methanol, orlistat, acetonitrile, deuterated methanol (MeOH-d₄), deuterated chloroform (CHCl₃-d), eriodictyol, naringenin, and quercetin were purchased from Sigma Aldrich. The solvents used for extraction (ethanol—EtOH), HPLC (acetonitrile—MeCN), and LC–MS (MeCN and water—H₂O) analyses were purchased from Merck. The ultra-pure water used for the HPLC analysis was from an in-house Milli-Q system.

TPA (99.9%), ISB (99.8%), and CHDM (99.8%) with 70 mole% trans-isomers were purchased from SK Chemicals (SKC; Suwon, Korea). NDA (99.8%) was purchased from BASF (Ludwigshafen, Germany). The ultra-pure (99%) catalyst (titanium-N-butoxide (TNBT) and thermal stabilizer (phosphorous acid) were used as received from Sigma Aldrich (Soul, South Korea). The high-purity monomers were used as received. High purity (99.9%) solvents, like deuterated chloroform (CHCl₃-d) (Sigma Aldrich, Soul, South Korea), deuterated trifluoroacetic acid (TFA-d) (Sigma Aldrich, Soul, South Korea), and o-chlorophenol (Sigma Aldrich, Soul, South Korea) used for the characterization of specimens, were used as received without any further purification.