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4 protocols using copper acetate

1

Copper-based Nanoparticle Anticancer Protocol

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Copper acetate was obtained from Macklin. Gallic acid (GA) was purchased from Kermel. Surfactant Aerosol OT was obtained from Alfa Aesar. Cisplatin was purchased from Shanghaiyuanye Bio-Technology Co., Ltd. l-arginine (l-Arg), NADH, glutathione (GSH) and H2O2 Assay Kit were obtained from Beijing Solarbio Science & Technology Co., Ltd. DSPE-PEG2000 was purchased from Top-Peptide Co., Ltd. BCA Kit, Lyso-Tracker Red, Hoechst 33342, DAPI, GSH Assay Kit, Calcein-AM/PI staining kit and Annexin V-FITC/PI were obtained from Beyotime Biotechnology. Charcoal-stripped fetal bovine serum (CS-FBS) was supplied by Hyclony. Nitric Oxide Assay Kit was obtained from Nanjing Jiancheng Bioengineering Institute. NO fluorescence probe (DAF-FM DA) was purchased from meilunbio. 4-hydroxyestradiol (4-OHE2) and 17β-estradiol (E2) were obtained from Cayman Chemical. PD98059 and Calpeptin were purchased from MCE. All other chemicals were provided by Sigma Aldrich (St. Louis, MO, USA) unless mentioned otherwise.
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2

Synthesis of Polyester Precursors

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Terephthalic acid (TPA),
ethylene glycol (EG), isosorbide, phenol, magnesium acetate, zinc
acetate, aluminum ethoxide, citric acid, and tetra-butyl ortho-titanate (TBOT) were purchased from Shanghai Aladdin Biochemical
Technology Co., Ltd. (Shanghai, China). Antimony trioxide, 1,1,2,2-tetrachloroethane
diantimony trioxide, antimony glycolate, hydroxydiacetyl iron, copper
acetate, bis(2-hydroxyethyl) terephthalate (BHET), and chloroform
were bought from Shanghai Macklin Biochemical Technology Co., Ltd.
(Shanghai, China). All materials were analytical reagents and used
without further purification. Nitrogen gases were obtained from Hangzhou
Jingong Special Gas Co., Ltd. (Hangzhou, China).
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3

Facile Synthesis of Copper-Titanium Oxide

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All chemicals are used directly without further purification. Copper acetate (Cu(CH3COO)2, AR, 99.0%), titanium butoxide (C16H36O4Ti, analytical reagent), potassium ferricyanide (K3[FeC6N6], AR, 99.5%), and ethanol (C2H5OH, 99.7%) are purchased from Macklin. Sodium hydroxide (NaOH, GR, 97%) is purchased from Aladdin. Ethylenediamine tetraacetic acid, sodium citrate, and sodium sulfate (Na2SO4, AR) are purchased from Shanghai Chemical Reagent.
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4

Synthesis and Characterization of Vanillin Derivatives

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2-methoxy-4-methylphenol, Vanillin, demethylVanillin, acetylacetone, tributyl borate, copper acetate, ethylene glycol, pyridine solution, boric anhydride, and n-butylamine, deuterium acetone, deuterium chloroform, 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Phosphoric acid (86%), tert-butyl alcohol, anhydrous Na2SO4, AlCl3, hydrochloric acid (HCl), ethyl acetate (EtOAc), petroleum ether (PE), ethanol, acetone, chloroform, hydrogen peroxide, and methanol were all analytical grade and purchased from Sinopharm Shanghai Chemical Reagent Company.
Lard used in this experiment was rendered in the laboratory and stored at −18 °C. Commercial soybean oil and potatoes were purchased from Wilmar International Limited (Shanghai, China). Soybean oil was purified using column chromatography (PE as eluent) to remove antioxidants and other polar components, especially tocopherols and other phenolic compounds in the oil.
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